Mass Spectrometry-Based Microbial Metabolomics: Techniques, Analysis, and Applications

  • Edward E. K. BaidooEmail author
  • Veronica Teixeira Benites
Part of the Methods in Molecular Biology book series (MIMB, volume 1859)


The demand for understanding the roles genes play in biological systems has steered the biosciences into the direction the metabolome, as it closely reflects the metabolic activities within a cell. The importance of the metabolome is further highlighted by its ability to influence the genome, transcriptome, and proteome. Consequently, metabolomic information is being used to understand microbial metabolic networks. At the forefront of this work is mass spectrometry, the most popular metabolomics measurement technique. Mass spectrometry-based metabolomic analyses have made significant contributions to microbiological research in the environment and human disease. In this chapter, we break down the technical aspects of mass spectrometry-based metabolomics and discuss its application to microbiological research.

Key words

Mass spectrometry Metabolomics Microbial LC-MS GC-MS CE-MS Metabolic quenching Metabolite extraction Data analysis Microbial communities Human disease 



The authors would also like to acknowledge that this work was part of the DOE Joint BioEnergy Institute ( supported by the US Department of Energy, Office of Science, Office of Biological and Environmental Research, through contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the US Department of Energy.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Edward E. K. Baidoo
    • 1
    • 2
    Email author
  • Veronica Teixeira Benites
    • 1
    • 2
  1. 1.Biological Systems and Engineering DivisionLawrence Berkeley National LaboratoryBerkeleyUSA
  2. 2.Joint BioEnergy InstituteEmeryvilleUSA

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