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Metabolomics Analysis of Leishmania by Capillary Electrophoresis and Mass Spectrometry

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Microbial Metabolomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1859))

Abstract

Capillary electrophoresis coupled to mass spectrometry is an analytical platform ideal for the analysis of ionic or polar metabolites. It constitutes a perfect complement to reversed-phase liquid chromatography, offering a good alternative to polar stationary phases where reproducibility is not guaranteed. Herein, we describe a robust standardized methodology for the fingerprinting analysis of Leishmania, a taxonomic genus which comprises more than 20 protozoa species.

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Acknowledgment

This work has been supported by the funding from the Spanish Ministry of Economy and Competitiveness (CTQ2014-55279-R).

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Correspondence to Ángeles López-Gonzálvez .

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Rojo, D., Barbas, C., López-Gonzálvez, Á. (2019). Metabolomics Analysis of Leishmania by Capillary Electrophoresis and Mass Spectrometry. In: Baidoo, E. (eds) Microbial Metabolomics. Methods in Molecular Biology, vol 1859. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8757-3_14

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  • DOI: https://doi.org/10.1007/978-1-4939-8757-3_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8756-6

  • Online ISBN: 978-1-4939-8757-3

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