Skip to main content

Metabolomics Analysis of Leishmania by Capillary Electrophoresis and Mass Spectrometry

  • Protocol
  • First Online:
Microbial Metabolomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1859))


Capillary electrophoresis coupled to mass spectrometry is an analytical platform ideal for the analysis of ionic or polar metabolites. It constitutes a perfect complement to reversed-phase liquid chromatography, offering a good alternative to polar stationary phases where reproducibility is not guaranteed. Herein, we describe a robust standardized methodology for the fingerprinting analysis of Leishmania, a taxonomic genus which comprises more than 20 protozoa species.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others


  1. Rojo D, Canuto GAB, Castilho-Martins EA et al (2015) A multiplatform metabolomic approach to the basis of antimonial action and resistance in Leishmania infantum. PLoS One 10:1–20

    Article  Google Scholar 

  2. Canuto GAB, Castilho-Martins EA, Tavares MFM et al (2014) Multi-analytical platform metabolomic approach to study miltefosine mechanism of action and resistance in Leishmania. Anal Bioanal Chem 406:3459–3476

    Article  CAS  Google Scholar 

  3. Alves-Ferreira EVC, Toledo JS, De Oliveira AHC et al (2015) Differential gene expression and infection profiles of cutaneous and mucosal Leishmania braziliensis isolates from the same patient. PLoS Negl Trop Dis 9:1–1

    Article  Google Scholar 

  4. Castilho-Martins EA, Canuto GAB, Muxel SM et al (2015) Capillary electrophoresis reveals polyamine metabolism modulation in Leishmania (Leishmania) amazonensis wild-type and arginase-knockout mutants under arginine starvation. Electrophoresis 36:2314–2323

    Article  CAS  Google Scholar 

  5. Godzien J, Ciborowski M, Angulo S et al (2013) From numbers to a biological sense: how the strategy chosen for metabolomics data treatment may affect final results. A practical example based on urine fingerprints obtained by LC-MS. Electrophoresis 34:2812–2826

    CAS  PubMed  Google Scholar 

  6. Korman A, Oh A, Banks D (2012) Statistical methods in metabolomics. In: Anisimova M (ed) Methods in molecular biology. Springer Science+Business Media, LLC, New York, pp 381–413

    Google Scholar 

  7. Steuer R, Morgenthal K, Weckwerth W et al (2007) A gentle guide to the analysis of metabolomic data. In: Weckwerth W (ed) Methods in molecular biology. Humana Press Inc., Totowa, pp 105–126

    Google Scholar 

  8. Vinaixa M, Schymanski EL, Neumann S et al (2016) Mass spectral databases for LC/MS- and GC/MS-based metabolomics: state of the field and future prospects. TrAC - Trends Anal Chem 78:23–35

    Article  CAS  Google Scholar 

  9. Gil de la Fuente A, Grace Armitage E, Otero A, et al (2017), Differentiating signals to make biological sense – a guide through databases for MS-based non-targeted metabolomics

    Google Scholar 

  10. Salek RM, Steinbeck C, Viant MR et al (2013) The role of reporting standards for metabolite annotation and identification in metabolomic studies. Gigascience 2

    Google Scholar 

  11. Godzien J, Armitage EG, Angulo S et al (2015) In-source fragmentation and correlation analysis as tools for metabolite identification exemplified with CE-TOF untargeted metabolomics. Electrophoresis 36:2188–2195

    Article  CAS  Google Scholar 

Download references


This work has been supported by the funding from the Spanish Ministry of Economy and Competitiveness (CTQ2014-55279-R).

Author information

Authors and Affiliations


Corresponding author

Correspondence to Ángeles López-Gonzálvez .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Rojo, D., Barbas, C., López-Gonzálvez, Á. (2019). Metabolomics Analysis of Leishmania by Capillary Electrophoresis and Mass Spectrometry. In: Baidoo, E. (eds) Microbial Metabolomics. Methods in Molecular Biology, vol 1859. Humana Press, New York, NY.

Download citation

  • DOI:

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8756-6

  • Online ISBN: 978-1-4939-8757-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics