Use of Liquid Chromatography–Mass Spectrometry-Based Metabolomics to Identify Biomarkers of Tuberculosis
Liquid chromatography–mass spectrometry (LC-MS) based metabolomics has proven to be a powerful analytical tool for biomarker screening. Here we describe two workflows which employ untargeted metabolomics to study serum biomarkers in tuberculosis patients. Expression profiles for samples of hydrophilic metabolites and hydrophobic metabolites (lipids) may be obtained by this method.
Key wordsLC-MS Metabolomics Serum Tuberculosis Hydrophilic metabolite Lipid
This work was supported by grants from the National Natural Science Foundation of China (7161007, 81430056, 31420103905, 21305005 and 31400695), the National Key Research and Development Program of China (No. 2016YFA0500302), and the Lam Chung Nin Foundation for Systems Biomedicine.
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