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Use of Liquid Chromatography–Mass Spectrometry-Based Metabolomics to Identify Biomarkers of Tuberculosis

  • Juntuo Zhou
  • Yuxin Yin
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1859)

Abstract

Liquid chromatography–mass spectrometry (LC-MS) based metabolomics has proven to be a powerful analytical tool for biomarker screening. Here we describe two workflows which employ untargeted metabolomics to study serum biomarkers in tuberculosis patients. Expression profiles for samples of hydrophilic metabolites and hydrophobic metabolites (lipids) may be obtained by this method.

Key words

LC-MS Metabolomics Serum Tuberculosis Hydrophilic metabolite Lipid 

Notes

Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (7161007, 81430056, 31420103905, 21305005 and 31400695), the National Key Research and Development Program of China (No. 2016YFA0500302), and the Lam Chung Nin Foundation for Systems Biomedicine.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Systems Biomedicine, School of Basic Medical SciencesPeking University Health Science CenterBeijingChina

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