Assessment of In Vivo Kidney Cell Death: Acute Kidney Injury

  • Wulf Tonnus
  • Moath Al-Mekhlafi
  • Christian Hugo
  • Andreas LinkermannEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1857)


The kidney has been studied as an organ to investigate cell death in vivo for a number of reasons. The unique vasculature that does not contain collateral vessels favors the kidney over other organs for the investigation of ischemia–reperfusion injury. Unilateral uretic obstruction has become the most prominently studied model for fibrosis with impact far beyond postrenal kidney injury. In addition, the tubular elimination mechanisms render the kidney susceptible to toxicity models, such as cisplatin-induced acute kidney injury. During trauma of skeletal muscles, myoglobulin deposition causes tubular cell death in the model of rhabdomyolysis-induced acute kidney injury. Here, we introduce these clinically relevant in vivo models of acute kidney injury (AKI) and critically review the protocols we use to effectively induce them.

Key words

Acute kidney injury (AKI) Kidney ischemia–reperfusion injury Unilateral ureteric obstruction Cisplatin-induced AKI Rhabdomyolysis-induced AKI 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Wulf Tonnus
    • 1
  • Moath Al-Mekhlafi
    • 1
  • Christian Hugo
    • 1
  • Andreas Linkermann
    • 1
    Email author
  1. 1.Division of Nephrology, Department of Internal Medicine IIIUniversity Hospital Carl Gustav Carus, Technische Universität DresdenDresdenGermany

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