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Protein Gel Detection and Imaging pp 255–258Cite as

Destaining Coomassie Brilliant Blue-Stained Sodium Dodecyl Sulfate–Polyacrylamide Protein Gels Using a Household Detergent

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1853))

Abstract

We report here a one-step method using the household detergent Vim Ultra to destain sodium dodecyl sulfate–polyacrylamide protein gels stained with Coomassie Brilliant Blue. This method, originally described by Pal and group, uses a 5% suspension of the detergent to destain gels efficiently. This method is cheap and user-friendly compared to the commonly used methanol–acetic acid–water containing destaining solvent.

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References

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Author information

Authors and Affiliations

  1. Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, University of Oklahoma, Oklahoma City, OK, USA

    Rachna Aggarwal

  2. Section of Endocrinology and Diabetes, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA

    Biji T. Kurien

  3. Department of Veterans Affairs Medical Center, Oklahoma City, OK, USA

    Biji T. Kurien

  4. Department of Arthritis and Clinical Immunology, Oklahoma City, OK, USA

    Biji T. Kurien

Authors
  1. Rachna Aggarwal
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  2. Biji T. Kurien
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Corresponding author

Correspondence to Biji T. Kurien .

Editor information

Editors and Affiliations

  1. Section of Endocrinology and Diabetes, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA

    Biji T. Kurien

  2. Section of Endocrinology and Diabetes, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA

    R. Hal Scofield

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Aggarwal, R., Kurien, B.T. (2018). Destaining Coomassie Brilliant Blue-Stained Sodium Dodecyl Sulfate–Polyacrylamide Protein Gels Using a Household Detergent. In: Kurien, B., Scofield, R. (eds) Protein Gel Detection and Imaging. Methods in Molecular Biology, vol 1853. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8745-0_29

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  • DOI: https://doi.org/10.1007/978-1-4939-8745-0_29

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8744-3

  • Online ISBN: 978-1-4939-8745-0

  • eBook Packages: Springer Protocols

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