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Microbial Carotenoids pp 221–237Cite as

Molecular Tools for Carotenogenesis Analysis in the Mucoral Mucor circinelloides

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1852))

Abstract

The carotene producer Mucor circinelloides is the fungus within the Mucoromycota phylum with the widest repertoire of molecular tools to manipulate its genome. The initial development of an effective procedure for genetic transformation and later improvements have resulted in an expansion of available tools, which include gene replacement, inactivation of gene expression by RNA silencing, gene overexpression, and functional genomics. Moreover, sequencing of its genome has given a definitive boost to these techniques making attainable the study of genes involved in many physiological or developmental processes, including carotenoid biosynthesis. Here, we describe in detail the latest molecular techniques currently used in M. circinelloides that have made it a valuable model for studying gene function within its phylum.

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Author information

Authors and Affiliations

  1. Departamento de Genética y Microbiología, Facultad de Biología, Universidad de Murcia, 30100, Murcia, Spain

    Francisco E. Nicolás, María Isabel Navarro-Mendoza, Carlos Pérez-Arques, Sergio López-García, Eusebio Navarro, Santiago Torres-Martínez & Victoriano Garre

Authors
  1. Francisco E. Nicolás
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  2. María Isabel Navarro-Mendoza
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  3. Carlos Pérez-Arques
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  4. Sergio López-García
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  5. Eusebio Navarro
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  6. Santiago Torres-Martínez
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  7. Victoriano Garre
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Corresponding author

Correspondence to Victoriano Garre .

Editor information

Editors and Affiliations

  1. Instituto de Biotecnología de León (INBIOTEC), Parque Científico de León, León, Spain

    Carlos Barreiro

  2. Department of Biotechnology, Crystal Pharma, A Subsidiary of Albany, Molecular Research Inc. (AMRI), Parque Tecnológico de León, León, Spain

    José-Luis Barredo

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Nicolás, F.E. et al. (2018). Molecular Tools for Carotenogenesis Analysis in the Mucoral Mucor circinelloides. In: Barreiro, C., Barredo, JL. (eds) Microbial Carotenoids. Methods in Molecular Biology, vol 1852. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8742-9_13

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  • DOI: https://doi.org/10.1007/978-1-4939-8742-9_13

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8741-2

  • Online ISBN: 978-1-4939-8742-9

  • eBook Packages: Springer Protocols

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