Abstract
Transient gene expression in human embryo kidney 293 (HEK293) cells is an established approach for the rapid production of large amounts of recombinant proteins (r-proteins). Milligram to gram quantities of r-proteins can be typically obtained within less than 10 days following transfection. In this chapter, we describe a simple and robust transfection process of suspension-growing human embryo kidney 293 cells using two commercially available serum-free media and polyethylenimine as the transfection reagent. This chapter provides examples for the production and purification of a his-tagged recombinant protein and two monoclonal antibodies.
Key words
- Transfection
- 293-6E
- Polyethylenimine
- Deacylated PEI
- Recombinant protein
- Suspension culture
- IMAC
- Protein-A
- Antibody
- Serum-free medium
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L’Abbé, D., Bisson, L., Gervais, C., Grazzini, E., Durocher, Y. (2018). Transient Gene Expression in Suspension HEK293-EBNA1 Cells. In: Hacker, D. (eds) Recombinant Protein Expression in Mammalian Cells. Methods in Molecular Biology, vol 1850. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8730-6_1
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DOI: https://doi.org/10.1007/978-1-4939-8730-6_1
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