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Considerations in the Use of Codon Optimization for Recombinant Protein Expression

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Recombinant Protein Expression in Mammalian Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1850))

Abstract

Codon optimization is a gene engineering approach that is commonly used for enhancing recombinant protein expression. This approach is possible because (1) degeneracy of the genetic code enables most amino acids to be encoded by multiple codons and (2) different mRNAs encoding the same protein can vary dramatically in the amount of protein expressed. However, because codon optimization potentially disrupts overlapping information encoded in mRNA coding regions, protein structure and function may be altered. This chapter discusses the use of codon optimization for various applications in mammalian cells as well as potential consequences, so that informed decisions can be made on the appropriateness of using this approach in each case.

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Authors and Affiliations

  1. The Scripps Research Institute, La Jolla, CA, USA

    Vincent P. Mauro

  2. Leidos, San Diego, CA, USA

    Stephen A. Chappell

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  1. Vincent P. Mauro
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  2. Stephen A. Chappell
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Correspondence to Vincent P. Mauro .

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Editors and Affiliations

  1. Protein Production and Structure Core Facility (PPSCF), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland

    David L. Hacker

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Mauro, V.P., Chappell, S.A. (2018). Considerations in the Use of Codon Optimization for Recombinant Protein Expression. In: Hacker, D. (eds) Recombinant Protein Expression in Mammalian Cells. Methods in Molecular Biology, vol 1850. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8730-6_18

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  • DOI: https://doi.org/10.1007/978-1-4939-8730-6_18

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