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Microfluidic Transfection for High-Throughput Mammalian Protein Expression

  • Kristina Woodruff
  • Sebastian J. Maerkl
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1850)

Abstract

Mammalian synthetic biology and cell biology would greatly benefit from improved methods for highly parallel transfection, culturing, and interrogation of mammalian cells. Transfection is routinely performed on high-throughput microarrays, but this setup requires manual cell culturing and precludes precise control over the cell environment. As an alternative, microfluidic transfection devices streamline cell loading and culturing. Up to 280 transfections can be implemented on the chip at high efficiency. The culturing environment is tightly regulated and chambers physically separate the transfection reactions, preventing cross-contamination. Unlike typical biological assays that rely on end-point measurements, the microfluidic chip can be integrated with high-content imaging, enabling the evaluation of cellular behavior and protein expression dynamics over time.

Key words

Microfluidics Transfection Microarrays DNA array Protein array Surface chemistry Protein expression Mammalian cells High-throughput screening Automated microscopy 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of Bioengineering, School of EngineeringÉcole Polytechnique Fédérale de LausanneLausanneSwitzerland

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