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Plant Small RNAs Responsive to Fungal Pathogen Infection

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Plant Pathogenic Fungi and Oomycetes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1848))

Abstract

Accumulating evidence indicates that small noncoding RNAs (sRNAs) can be transferred across species for interkingdom communication. In addition to the artificial transgene-derived small interfering RNAs (siRNAs), endogenous microRNAs (miRNAs) can also influence interacting organisms to execute a regulatory function. For instance, we have recently found that, in response to infection with Verticillium dahliae (V. dahliae), cotton plants increase accumulation of miR166 and miR159, which can be exported to the fungal hyphae for specific silencing of virulence genes. These findings suggest a great potential for applying interkingdom mobile miRNAs for crop protection against fungal pathogens. The methods described here provide an approach to identify plant miRNAs and their potential targets in invading fungal pathogens, which will help in revealing the underlying mechanisms of these crosstalk phenomena.

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Acknowledgments

This work was supported by grants from the Natural Science Foundation of China (31730078 to H.S.G. and 31700131 to Y.J.).

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Authors and Affiliations

  1. State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China

    Yun Jin & Hui-Shan Guo

  2. College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China

    Hui-Shan Guo

Authors
  1. Yun Jin
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  2. Hui-Shan Guo
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Corresponding author

Correspondence to Hui-Shan Guo .

Editor information

Editors and Affiliations

  1. Department of Microbiology and Plant Pathology, University of California Riverside, Riverside, CA, USA

    Wenbo Ma

  2. Oregon State University, Corvallis, OR, USA

    Thomas Wolpert

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Jin, Y., Guo, HS. (2018). Plant Small RNAs Responsive to Fungal Pathogen Infection. In: Ma, W., Wolpert, T. (eds) Plant Pathogenic Fungi and Oomycetes. Methods in Molecular Biology, vol 1848. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8724-5_6

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  • DOI: https://doi.org/10.1007/978-1-4939-8724-5_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8723-8

  • Online ISBN: 978-1-4939-8724-5

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