Methods for Preparing Cryo-EM Grids of Large Macromolecular Complexes
The recent resolution revolution in cryo-electron microscopy has generated a huge interest in the technique for determining atomic resolution structures of large and dynamic macromolecular complexes that are intractable to crystallography and NMR. A key element of success in cryo-EM is the quality of the specimen vitrified on the cryo-EM grid. In this chapter we outline methods for cryo-EM grid sample preparation.
Key wordsCryo-electron microscopy (cryo-EM) Cryo-EM grids APC/C Multi-subunit complexes
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