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Methods for Preparing Cryo-EM Grids of Large Macromolecular Complexes

  • Leifu Chang
  • David BarfordEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1844)

Abstract

The recent resolution revolution in cryo-electron microscopy has generated a huge interest in the technique for determining atomic resolution structures of large and dynamic macromolecular complexes that are intractable to crystallography and NMR. A key element of success in cryo-EM is the quality of the specimen vitrified on the cryo-EM grid. In this chapter we outline methods for cryo-EM grid sample preparation.

Key words

Cryo-electron microscopy (cryo-EM) Cryo-EM grids APC/C Multi-subunit complexes 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.MRC Laboratory of Molecular BiologyCambridgeUK

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