Small-Angle X-Ray Scattering for the Study of Proteins in the Ubiquitin Pathway

  • Jean-François TrempeEmail author
  • Kalle GehringEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1844)


Small-angle X-ray scattering (SAXS) is an invaluable complement to other biophysical methods used to interrogate the structure and dynamics of proteins. Here, we describe the standard experimental protocol used in our laboratory to analyze proteins in the ubiquitin pathway. The method addresses buffer selection, data collection using an in-house X-ray source, diagnostic tests to assess data quality, and computational approaches to interpret SAXS data.

Key words

X-ray scattering SAXS Ubiquitin Kratky plot Guinier analysis Pair-distance distribution function 


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Pharmacology and TherapeuticsMcGill UniversityMontréalCanada
  2. 2.Department of BiochemistryMcGill UniversityMontréalCanada

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