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Computational Methods for Studying the Plant Nucleus

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The LINC Complex

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1840))

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Abstract

The analysis of nuclear envelope components and their function has recently been progressed by the use of computational methods of analysis. The methods in this chapter provided by members of the International Plant Nucleus Consortium address the identification of novel nuclear envelope proteins and the study of structure and mobility of the nucleus. DORY2 is an upgrade of the KASH-finder DORY, and NucleusJ is used to characterize the three-dimensional structure of the nucleus in light microscope images. Finally, a method is provided for analysis of the migration of the nucleus, a key technique for exploring the function of plant nuclear proteins.

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Acknowledgments

X.Z. is supported by the Gordon and Betty Moore Foundation through Grant GBMF2550.06. Work in I.M.’s lab is funded by the National Science Foundation. I.M. would like to thank Norman Groves and Anna Newman-Griffis, OSU for “test running” DORY2. K.T. is supported by the Grant-in-Aid for Scientific Research from JSPS (No. 26711017 and 15K14545). The authors are participants in COST Action CA 16212 ‘In Depth’.

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Author notes

  1. Axel Poulet

    Present address: Department of Biostatistics and Bioinformatics, Rollins School of Public Health, Emory University, Atlanta, GA, USA

  2. Xiao Zhou

    Present address: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA

Authors and Affiliations

  1. Laboratoire GReD, CNRS, INSERM, Université Clermont Auvergne, Clermont-Ferrand, F-63000, France

    Axel Poulet & Christophe Tatout

  2. Faculty of Health and Life Sciences, Department of Biological and Medical Sciences, Oxford Brookes University, Headington Campus, Oxford, OX3 0BP, UK

    Axel Poulet, Iris Meier, Katja Graumann & David E. Evans

  3. Department of Molecular Genetics, The Ohio State University, 318 West 12th Avenue, Columbus, OH 43210, OH, USA

    Xiao Zhou & Iris Meier

  4. Department of Environmental and Life Sciences, School of Food and Nutritional Sciences, University of Shizuoka, Shizuoka, 422-8526, Japan

    Kentaro Tamura

Authors
  1. Axel Poulet
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  2. Xiao Zhou
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  3. Kentaro Tamura
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  4. Iris Meier
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  5. Christophe Tatout
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  6. Katja Graumann
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  7. David E. Evans
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Corresponding author

Correspondence to David E. Evans .

Editor information

Editors and Affiliations

  1. Department of Pathology and Cell Biology, Columbia University, New York, New York, USA

    Gregg G. Gundersen

  2. Department of Medicine and Department of Pathology and Cell Biology, Columbia University, New York, New York, USA

    Howard J. Worman

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Poulet, A. et al. (2018). Computational Methods for Studying the Plant Nucleus. In: Gundersen, G., Worman, H. (eds) The LINC Complex. Methods in Molecular Biology, vol 1840. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8691-0_15

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  • DOI: https://doi.org/10.1007/978-1-4939-8691-0_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8690-3

  • Online ISBN: 978-1-4939-8691-0

  • eBook Packages: Springer Protocols

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