Infant Mouse Model of Vibrio cholerae Infection and Colonization

  • Jyl S. MatsonEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1839)


Cholera is an epidemic diarrheal disease caused by Vibrio cholerae that continues to cause significant morbidity and mortality in many parts of the world. Several different animal models have been used by scientists over the years to study the pathogenesis of cholera. However, the most commonly used is the infant (suckling) mouse model, which has been found to replicate important aspects of human intestinal colonization. Here we present a detailed protocol for using the infant mouse model to assess the colonization of V. cholerae strains using a competition assay.

Key words

Vibrio cholerae Mouse model Competitive index Colonization 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Medical Microbiology and ImmunologyUniversity of Toledo College of Medicine and Life SciencesToledoUSA

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