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Utilization of Vibrio cholerae as a Model Organism to Screen Natural Product Libraries for Identification of New Antibiotics

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Book cover Vibrio Cholerae

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1839))

Abstract

The development of antibiotic-resistant bacteria requires increasing research efforts in drug discovery. Vibrio cholerae can be utilized as a model gram-negative enteric pathogen in high- and medium-throughput screening campaigns to identify antimicrobials with different modes of action. In this chapter, we describe methods for the optimal growth of V. cholerae in 384-well plates, preparation of suitable microtiter natural product sample libraries, as well as their screening using measurements of bacterial density and activity of type II secretion-dependent protease as readouts. Concomitant LC-MS/MS profiling and spectral data networking of assay sample libraries facilitate dereplication of putative known and/or nuisance compounds and efficient prioritization of samples containing putative new natural products for further investigation.

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Acknowledgments

This work was funded by the start-up funds to A.E.S. and an Oregon Sea Grant under award number NA10OAR4170059 (project number R/BT-48) from the National Oceanic and Atmospheric Administration’s National Sea Grant College Program to K.L.M. and A.E.S.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR, USA

    Aleksandra E. Sikora, Richard Tehan & Kerry McPhail

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  1. Aleksandra E. Sikora
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  2. Richard Tehan
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  3. Kerry McPhail
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Correspondence to Aleksandra E. Sikora .

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Editors and Affiliations

  1. Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, Oregon, USA

    Aleksandra E. Sikora

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Sikora, A.E., Tehan, R., McPhail, K. (2018). Utilization of Vibrio cholerae as a Model Organism to Screen Natural Product Libraries for Identification of New Antibiotics. In: Sikora, A. (eds) Vibrio Cholerae. Methods in Molecular Biology, vol 1839. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8685-9_12

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  • DOI: https://doi.org/10.1007/978-1-4939-8685-9_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8684-2

  • Online ISBN: 978-1-4939-8685-9

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