Laboratory Culturing Techniques and Maintenance of Vibrio cholerae

  • Kyle D. Brumfield
  • Bailey M. Carignan
  • Mike S. SonEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1839)


Cholera is a severe diarrheal disease caused by the consumption of food or water contaminated with the aquatic gram-negative bacterium Vibrio cholerae. Infected hosts will experience vomiting and severe watery diarrhea and if not treated properly will ultimately succumb to death by dehydration. Due to the global prevalence and severity of cholera, V. cholerae has been extensively studied in both environmental and laboratory settings. Herein, we describe proper V. cholerae maintenance, in addition to classical and El Tor biotype culturing in a laboratory setting.

Key words

Vibrio cholerae Laboratory Growth Maintenance Virulence inducing 



Research reported in this publication was supported by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant number P20GM103506.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Kyle D. Brumfield
    • 1
  • Bailey M. Carignan
    • 1
  • Mike S. Son
    • 1
    Email author
  1. 1.Department of Biological SciencesPlymouth State UniversityPlymouthUSA

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