NetoVIR: Modular Approach to Customize Sample Preparation Procedures for Viral Metagenomics

  • Nádia Conceição-Neto
  • Kwe Claude Yinda
  • Marc Van Ranst
  • Jelle MatthijnssensEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1838)


The democratization of next-generation sequencing (NGS) technologies has enabled scientists to explore the diversity of microbial life in various ecological niches in an unpreceded depth. The role of viruses as a key player in health and disease is becoming increasingly clear. To address the need for an up scalable, reproducible protocol to purify RNA and DNA viruses from a sample, we describe our optimized method. The Novel Enrichment Technique Of Viromes (NetoVIR) allows researchers to attain a fast, reproducible, and high-throughput sample preparation protocol for NGS gut viromics studies. With appropriate prior homogenization steps, this protocol can be extended to any biological samples.

Key words

Viromics Sample preparation Next-generation sequencing Virome 



We would like to thank Lila Close for insightful tips and tricks.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Nádia Conceição-Neto
    • 1
    • 2
  • Kwe Claude Yinda
    • 1
    • 2
  • Marc Van Ranst
    • 2
  • Jelle Matthijnssens
    • 1
    Email author
  1. 1.Laboratory of Viral Metagenomics, Department of Microbiology and Immunology, Rega InstituteKU Leuven – University of LeuvenLeuvenBelgium
  2. 2.Laboratory of Clinical Virology, Department of Microbiology and Immunology, Rega InstituteKU Leuven – University of LeuvenLeuvenBelgium

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