Abstract
This chapter proposes a simple, standardized protocol for generating RNA viromes from complex host-associated biological samples of various origins. Compared to other existing protocols to generate RNA viromes, this protocol preserves the infectivity of viral particles and allows for downstream applications such as viral characterization and isolation tests.
Key words
- Virome
- Arthropods
- Metagenomics
- RNA viruses
- Sucrose density gradient
- Viral zoonosis
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Acknowledgments
This work was supported by a grant from the Agence Nationale de la Recherche number ANR-13-JSV6-0004 to CD.
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Monteil-Bouchard, S., Temmam, S., Desnues, C. (2018). Protocol for Generating Infectious RNA Viromes from Complex Biological Samples. In: Moya, A., Pérez Brocal, V. (eds) The Human Virome. Methods in Molecular Biology, vol 1838. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8682-8_3
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DOI: https://doi.org/10.1007/978-1-4939-8682-8_3
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