Protocol for Generating Infectious RNA Viromes from Complex Biological Samples

  • Sonia Monteil-Bouchard
  • Sarah Temmam
  • Christelle DesnuesEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1838)


This chapter proposes a simple, standardized protocol for generating RNA viromes from complex host-associated biological samples of various origins. Compared to other existing protocols to generate RNA viromes, this protocol preserves the infectivity of viral particles and allows for downstream applications such as viral characterization and isolation tests.

Key words

Virome Arthropods Metagenomics RNA viruses Sucrose density gradient Viral zoonosis 



This work was supported by a grant from the Agence Nationale de la Recherche number ANR-13-JSV6-0004 to CD.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Sonia Monteil-Bouchard
    • 1
  • Sarah Temmam
    • 2
  • Christelle Desnues
    • 1
    Email author
  1. 1.Unité de Recherche sur les Maladies Infectieuses Tropicales Emergentes (URMITE), IHU Méditerranée Infection, Assistance-Publique des Hôpitaux de Marseille, Aix-Marseille Université, CNRS 7278, IRD 198, INSERM1095MarseilleFrance
  2. 2.Biology of Infection Unit, Laboratory of Pathogen Discovery, Institut PasteurINSERM U1117ParisFrance

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