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Live Imaging of Influenza Viral Ribonucleoproteins Using Light-Sheet Microscopy

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Influenza Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1836))

Abstract

Influenza viruses exhibit a complex life cycle that is still poorly understood. It involves independent replication of each of the eight segments that make up its genome and subsequent coordinated assembly as they egress from the host cell. Fast, time-resolved volumetric live cell imaging offers a powerful tool for understanding the various host mechanisms hijacked by the virus. Here, we describe the methods necessary for generating influenza viruses that carry a fluorescently tagged polymerase complex, infection of biologically relevant cells with these viruses, and finally protocols for live cell imaging and analysis.

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Authors and Affiliations

  1. Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    Amar R. Bhagwat, Valerie Le Sage & Seema S. Lakdawala

Authors
  1. Amar R. Bhagwat
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  2. Valerie Le Sage
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  3. Seema S. Lakdawala
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Corresponding author

Correspondence to Seema S. Lakdawala .

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Editors and Affiliations

  1. School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK

    Yohei Yamauchi

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Bhagwat, A.R., Le Sage, V., Lakdawala, S.S. (2018). Live Imaging of Influenza Viral Ribonucleoproteins Using Light-Sheet Microscopy. In: Yamauchi, Y. (eds) Influenza Virus. Methods in Molecular Biology, vol 1836. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8678-1_15

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  • DOI: https://doi.org/10.1007/978-1-4939-8678-1_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8677-4

  • Online ISBN: 978-1-4939-8678-1

  • eBook Packages: Springer Protocols

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