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Bacterial Chromatin pp 161–176Cite as

Dynamic Light Scattering of DNA-Ligand Complexes

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1837))

Abstract

Dynamic Light Scattering (DLS) enables the characterization of sizes and electrokinetic properties of colloids, polymers, macromolecules. DNA is a charged semiflexible polyelectrolyte, which is condensed or compacted by counterions, proteins, and other condensing agents in processes such as chromosome compaction and gene therapeutic applications. DNA condensation is closely related to charge screening, since packaging requires effective neutralization of its surface negative charges. In this chapter, we describe in detail the protocol for DLS of DNA-ligand complexes. As an example, we describe data for condensation of DNA by chitosan and the measurement of size, zeta potential, and electrophoretic mobility of the DNA-ligand complex by DLS.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 11574232, 11274245, 11304232).

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Authors and Affiliations

  1. Department of Physics, Wenzhou University, Wenzhou, China

    Guangcan Yang & Yanwei Wang

Authors
  1. Guangcan Yang
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  2. Yanwei Wang
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Corresponding author

Correspondence to Guangcan Yang .

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Editors and Affiliations

  1. Leiden Institute of Chemistry and Centre for Microbial Cell Biology, Leiden University, Leiden, The Netherlands

    Remus T. Dame

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Yang, G., Wang, Y. (2018). Dynamic Light Scattering of DNA-Ligand Complexes. In: Dame, R. (eds) Bacterial Chromatin. Methods in Molecular Biology, vol 1837. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8675-0_10

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  • DOI: https://doi.org/10.1007/978-1-4939-8675-0_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8674-3

  • Online ISBN: 978-1-4939-8675-0

  • eBook Packages: Springer Protocols

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