Generation of Ultra-Small PLGA Nanoparticles by Sequential Centrifugation

  • Xingwang Wu
  • Jiangbing Zhou
  • Toral R. PatelEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1831)


Direct, local delivery of polymer nanoparticles to the brain is a promising strategy to bypass the blood-brain barrier (BBB) and safely deliver a large therapeutic payload. However, even with the aid of convection-enhanced delivery (CED) techniques, this approach has been limited by the inability to fabricate appropriately sized polymer nanoparticles. Here, we outline a versatile and efficient method for producing polymer nanoparticles that are <100 nm in diameter and can be delivered to the brain via CED.

Key words

Nanoparticle Polymer Convection-enhanced delivery PLGA Brain Blood-brain barrier 



This work was supported by NIH Grants NS095817, NS095147, the State of Connecticut (J.Z.) and a discovery grant from the American Brain Tumor Association (J.Z.).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of NeurosurgeryYale UniversityNew HavenUSA
  2. 2.Department of RadiologyThe First Affiliated Hospital of Anhui Medical UniversityHefeiChina
  3. 3.Department of Neurological SurgeryUT Southwestern Medical CenterDallasUSA

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