Advertisement

Generation of Ultra-Small PLGA Nanoparticles by Sequential Centrifugation

  • Xingwang Wu
  • Jiangbing Zhou
  • Toral R. PatelEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1831)

Abstract

Direct, local delivery of polymer nanoparticles to the brain is a promising strategy to bypass the blood-brain barrier (BBB) and safely deliver a large therapeutic payload. However, even with the aid of convection-enhanced delivery (CED) techniques, this approach has been limited by the inability to fabricate appropriately sized polymer nanoparticles. Here, we outline a versatile and efficient method for producing polymer nanoparticles that are <100 nm in diameter and can be delivered to the brain via CED.

Key words

Nanoparticle Polymer Convection-enhanced delivery PLGA Brain Blood-brain barrier 

Notes

Acknowledgments

This work was supported by NIH Grants NS095817, NS095147, the State of Connecticut (J.Z.) and a discovery grant from the American Brain Tumor Association (J.Z.).

References

  1. 1.
    Patel T, Zhou J, Piepmeier JM, Saltzman WM (2012) Polymeric nanoparticles for drug delivery to the central nervous system. Adv Drug Deliv Rev 64:701–705CrossRefPubMedGoogle Scholar
  2. 2.
    Zhou J, Atsina KB, Himes BT, Strohbehn GW, Saltzman WM (2012) Novel delivery strategies for glioblastoma. Cancer J 18:89–99CrossRefPubMedGoogle Scholar
  3. 3.
    Zhou J, Patel TR, Fu M, Bertram JP, Saltzman WM (2012) Octa-functional PLGA nanoparticles for targeted and efficient siRNA delivery to tumors. Biomaterials 33:583–591CrossRefPubMedGoogle Scholar
  4. 4.
    Thorne RG, Nicholson C (2006) In vivo diffusion analysis with quantum dots and dextrans predicts the width of brain extracellular space. Proc Natl Acad Sci U S A 103:5567–5572CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Hobbs SK, Monsky WL, Yuan F, Roberts WG, Griffith L, Torchilin VP, Jain RK (1998) Regulation of transport pathways in tumor vessels: role of tumor type and microenvironment. Proc Natl Acad Sci U S A 95:4607–4612CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Zhou J, Patel TR, Sirianni RW, Strohbehn G, Zheng M-Q, Duong N, Schafbauer T, Huttner AJ, Huang Y, Carson RE, Zhang Y, Sullivan DJ Jr, Piepmeier JM, Saltzman WM (2013) Highly penetrative, drug-loaded nanocarriers improve treatment of glioblastoma. Proc Natl Acad Sci U S A 110:11751–11756CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of NeurosurgeryYale UniversityNew HavenUSA
  2. 2.Department of RadiologyThe First Affiliated Hospital of Anhui Medical UniversityHefeiChina
  3. 3.Department of Neurological SurgeryUT Southwestern Medical CenterDallasUSA

Personalised recommendations