Utilizing the Lung as a Model to Study Nanoparticle-Based Drug Delivery Systems

  • Dylan K. McDaniel
  • Veronica M. Ringel-Scaia
  • Sheryl L. Coutermarsh-Ott
  • Irving C. AllenEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1831)


Intranasal administration is a highly effective route for drug delivery and biodistribution studies. Indeed, this route of delivery has become the method of choice to distribute diverse pharmacological agents both locally and systemically. In the majority of preclinical animal models and in human patients, intranasal administration is the preferred method to deliver therapeutic agents to the airways and lungs. However, issues with drug stability and controlled release in the respiratory tract are common problems with many therapeutic agents. Nanoparticle delivery via intranasal administration has tremendous potential to circumvent these common issues. Over the past 30 years nanoparticles have gained increased interest as therapeutic delivery vehicles and as tools for improved bioimaging. Integral to the success of nanoparticles in drug delivery and biodistribution is the utilization of mouse models to characterize therapeutic strategies under physiologically relevant in situ conditions. Here, we describe a model of nanoparticle administration to the lungs utilizing intranasal administration and discuss a variety of highly useful techniques to evaluate nanoparticle up-take, biodistribution, and immune response. While these protocols have been optimized for intranasal administration of common fluorescently labeled nanoparticles, they can be applied to any nanoparticle or drug delivery system of interest targeting the lungs and airways.

Key words

Biodistribution Intranasal administration Inflammation Airway Flow cytometry 



Funding for this work was provided by the Virginia Tech Institute for Critical Technology and Applied Science and the Virginia Maryland College of Veterinary Medicine. Nanoparticles utilized in this protocol were provided by Prof. Richey M. Davis from the Virginia Tech Department of Chemical Engineering.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Dylan K. McDaniel
    • 1
  • Veronica M. Ringel-Scaia
    • 2
  • Sheryl L. Coutermarsh-Ott
    • 1
  • Irving C. Allen
    • 1
    • 2
    • 3
    Email author
  1. 1.Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary MedicineVirginia TechBlacksburgUSA
  2. 2.Graduate Program in Translational Biology, Medicine, and HealthVirginia TechBlacksburgUSA
  3. 3.Department of Biomedical SciencesCarilion School of Medicine, Virginia TechRoanokeUSA

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