Synthesis of Cationic Polymer Libraries for Gene Delivery Using Diglycidyl Ethers

  • Jacob Elmer
  • Thrimoorthy Potta
  • Kaushal RegeEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1831)


Gene therapy has the potential to cure many different genetic diseases, if safe and effective gene delivery vectors can be developed. This chapter describes protocols for the synthesis of novel polymers using diglycidyl ether and diamine or polyamine monomers for transgene delivery and expression. The resulting poly (amino ethers) are able to transfect a higher number of cells, with lower cytotoxicity than other commercially available polymers (e.g., Polyethyleneimine, PEI).

Key words

Polymers Gene therapy Gene delivery Polyplex Aminoglycosides Diglycidyl ethers 



We are grateful to the NIH/NIGMS (Grant 1R01GM093229-01A1) for financial support of this study. Chemical structures were drawn using ChemDraw based on those available on the MilliporeSigma website.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Chemical EngineeringVillanova UniversityVillanovaUSA
  2. 2.Chemical Engineering, School for Engineering of Matter, Transport and EnergyArizona State UniversityTempeUSA

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