Synthesis of Cationic Polymer Libraries for Gene Delivery Using Diglycidyl Ethers
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Gene therapy has the potential to cure many different genetic diseases, if safe and effective gene delivery vectors can be developed. This chapter describes protocols for the synthesis of novel polymers using diglycidyl ether and diamine or polyamine monomers for transgene delivery and expression. The resulting poly (amino ethers) are able to transfect a higher number of cells, with lower cytotoxicity than other commercially available polymers (e.g., Polyethyleneimine, PEI).
Key wordsPolymers Gene therapy Gene delivery Polyplex Aminoglycosides Diglycidyl ethers
We are grateful to the NIH/NIGMS (Grant 1R01GM093229-01A1) for financial support of this study. Chemical structures were drawn using ChemDraw based on those available on the MilliporeSigma website.