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Ectopic Vascular Induction in Arabidopsis Cotyledons for Sequential Analysis of Phloem Differentiation

  • Alif Meem Nurani
  • Yuki KondoEmail author
  • Hiroo FukudaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1830)

Abstract

Vascular system is vital for the transport of water and nutrients as well as for providing mechanical support in many land plants. Plant transcription factors play a central role in regulating vascular development downstream of hormones and peptide signaling pathways. Particularly, cell culture systems have contributed to isolating such key transcription factors for xylem differentiation. However, there had been no efficient systems that can mimic phloem differentiation in the model plant Arabidopsis, preventing the identification of phloem-related transcription factors. We have recently established Vascular cell Induction culture System Using Arabidopsis Leaves (VISUAL), which concomitantly generates both xylem and phloem cells in the cotyledon of Arabidopsis. This system can be used to take a closer look at the bi-directional differentiation mechanism of (pro)cambial cells into xylem and phloem cells. Here, we report the methods of microscopic, genetic, and molecular analysis using VISUAL, which can help in decrypting the transcriptional networks that regulate vascular cell differentiation.

Key words

Vascular induction Arabidopsis Differentiation Procambium Cambium Xylem Phloem Cell-sorting Tissue culture 

Notes

Acknowledgments

This work was supported by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (17H06476 to Y.K. and 15H05958 to H.F.), from the Japan Society for the Promotion of Science (17H05008 to Y.K. and 16H06377 to H.F.) and from Naito Foundation to H.F.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biological Sciences, Graduate School of ScienceThe University of TokyoTokyoJapan

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