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Plastids pp 273–278Cite as

Mapping Plastid Transcript Population by Circular Reverse Transcription Polymerase Chain Reaction

Part of the Methods in Molecular Biology book series (MIMB,volume 1829)

Abstract

During evolution of photosynthetic organisms, the genetic information provided by the internalized cyanobacteria has been transferred to the nucleus. The small genome kept by the chloroplast, the so-called plastome, displays a complex organization, comprising operons under the control of multiples promoters. In addition, polycistronic transcripts undergo multiple processing events, thus generating a complex population of mRNAs from a single gene. This chapter describes a method to investigate the diversity of the mRNA population from a single gene by circular RT-PCR. The protocol provided here allows for the simultaneous mapping of both 5′ and 3′ ends of the same RNA molecule.

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References

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Acknowledgments

This work was supported by the French National Research Agency (ANR-13-ADAP-0008 Reglisse and ANR-10-LABEX-04 GRAL Labex, Grenoble Alliance for Integrated Structural Cell Biology).

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Correspondence to Florence Courtois .

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Courtois, F., Merendino, L. (2018). Mapping Plastid Transcript Population by Circular Reverse Transcription Polymerase Chain Reaction. In: Maréchal, E. (eds) Plastids. Methods in Molecular Biology, vol 1829. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8654-5_18

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  • DOI: https://doi.org/10.1007/978-1-4939-8654-5_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8653-8

  • Online ISBN: 978-1-4939-8654-5

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