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Plastids pp 273–278Cite as

Mapping Plastid Transcript Population by Circular Reverse Transcription Polymerase Chain Reaction

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1829))

Abstract

During evolution of photosynthetic organisms, the genetic information provided by the internalized cyanobacteria has been transferred to the nucleus. The small genome kept by the chloroplast, the so-called plastome, displays a complex organization, comprising operons under the control of multiples promoters. In addition, polycistronic transcripts undergo multiple processing events, thus generating a complex population of mRNAs from a single gene. This chapter describes a method to investigate the diversity of the mRNA population from a single gene by circular RT-PCR. The protocol provided here allows for the simultaneous mapping of both 5′ and 3′ ends of the same RNA molecule.

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References

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Acknowledgments

This work was supported by the French National Research Agency (ANR-13-ADAP-0008 Reglisse and ANR-10-LABEX-04 GRAL Labex, Grenoble Alliance for Integrated Structural Cell Biology).

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Authors and Affiliations

  1. Centre National de la Recherche Scientifique, Institute of Plant Sciences Paris Saclay, Institut National de la Recherche Agronomique, Université Paris-Sud, Université Evry, Université Paris-Saclay, Paris Diderot, Sorbonne Paris-Cité, Gif sur Yvette Cedex, France

    Livia Merendino

  2. Laboratoire de Physiologie Cellulaire et Végétale, Centre National de la Recherche Scientifique, Institut National Recherche Agronomique, Commissariat à l’Energie Atomique et aux Energies Alternatives, CEA Grenoble, UMR5168, Université Grenoble Alpes, Grenoble, France

    Florence Courtois & Livia Merendino

Authors
  1. Florence Courtois
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  2. Livia Merendino
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Corresponding author

Correspondence to Florence Courtois .

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Editors and Affiliations

  1. Laboratoire de Physiologie Cellulaire et Végétale, Centre National de la Recherche Scientifique, Commissariat à l’Energie Atomique et aux Energies Alternatives, CEA Grenoble, Institut National Recherche Agronomique, UMR5168, Université Grenoble Alpes, Grenoble, France

    Eric Maréchal

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Courtois, F., Merendino, L. (2018). Mapping Plastid Transcript Population by Circular Reverse Transcription Polymerase Chain Reaction. In: Maréchal, E. (eds) Plastids. Methods in Molecular Biology, vol 1829. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8654-5_18

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  • DOI: https://doi.org/10.1007/978-1-4939-8654-5_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8653-8

  • Online ISBN: 978-1-4939-8654-5

  • eBook Packages: Springer Protocols

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