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Antibody Engineering pp 491–503Cite as

Selection and Use of Intracellular Antibodies

Part of the Methods in Molecular Biology book series (MIMB,volume 1827)

Abstract

Intracellularly expressed recombinant antibodies, or intrabodies, are powerful tools for cell biology studies as well as therapeutic applications. Cell biologists use them to either block the intracellular antibody target or to image endogenous target dynamics. We describe here methods to select recombinant antibodies from antibody phage display libraries and to subsequently express them as fluorescent intrabodies.

Key words

  • Phage display
  • Intrabody
  • Live cell imaging

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  • DOI: 10.1007/978-1-4939-8648-4_25
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Acknowledgments

This work was supported by the Centre National de la Recherche Scientifique (CNRS), the Institut Curie, and the LaBex CelTisPhyBio (ANR-10-LBX-0038 part of the IDEX PSL no ANR-10-IDEX-0001-02) and by grants from the Agence National de la Recherche (ANR), the Institut National du Cancer (INCa), and the Agence Nationale de Recherche sur le SIDA (ANRS).

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Authors and Affiliations

  1. CNRS, UMR144, Paris, France

    Sandrine Moutel & Franck Perez

  2. Institut Curie, PSL Research University, Paris, France

    Sandrine Moutel & Franck Perez

  3. Laboratoire de Biochimie, ESPCI Paris, PSL Research University, CNRS UMR8231 Chimie Biologie Innovation, Paris, France

    Clément Nizak

Authors
  1. Sandrine Moutel
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  2. Clément Nizak
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  3. Franck Perez
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Corresponding author

Correspondence to Franck Perez .

Editor information

Editors and Affiliations

  1. Immunology Division, Garvan Institute of Medical Research, Darlinghurst, NSW, Australia

    Damien Nevoltris

  2. Institut Paoli-Calmettes, CRCM, Aix Marseille University, CNRS, INSERM, Marseille, France

    Patrick Chames

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Moutel, S., Nizak, C., Perez, F. (2018). Selection and Use of Intracellular Antibodies. In: Nevoltris, D., Chames, P. (eds) Antibody Engineering. Methods in Molecular Biology, vol 1827. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8648-4_25

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  • DOI: https://doi.org/10.1007/978-1-4939-8648-4_25

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8647-7

  • Online ISBN: 978-1-4939-8648-4

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