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Selection and Use of Intracellular Antibodies

Part of the Methods in Molecular Biology book series (MIMB,volume 1827)


Intracellularly expressed recombinant antibodies, or intrabodies, are powerful tools for cell biology studies as well as therapeutic applications. Cell biologists use them to either block the intracellular antibody target or to image endogenous target dynamics. We describe here methods to select recombinant antibodies from antibody phage display libraries and to subsequently express them as fluorescent intrabodies.

Key words

  • Phage display
  • Intrabody
  • Live cell imaging

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  • DOI: 10.1007/978-1-4939-8648-4_25
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This work was supported by the Centre National de la Recherche Scientifique (CNRS), the Institut Curie, and the LaBex CelTisPhyBio (ANR-10-LBX-0038 part of the IDEX PSL no ANR-10-IDEX-0001-02) and by grants from the Agence National de la Recherche (ANR), the Institut National du Cancer (INCa), and the Agence Nationale de Recherche sur le SIDA (ANRS).

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Correspondence to Franck Perez .

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Moutel, S., Nizak, C., Perez, F. (2018). Selection and Use of Intracellular Antibodies. In: Nevoltris, D., Chames, P. (eds) Antibody Engineering. Methods in Molecular Biology, vol 1827. Humana Press, New York, NY.

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8647-7

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