Abstract
Single-domain antibody (e.g., Nanobody, VHH antibody) is a promising scaffold for therapeutic and diagnostic reagents. To expand the range of target molecules, in vitro selection using cell-free display technologies such as cDNA display is useful and powerful because of their huge libraries and robust stability. We provide technical details for in vitro selection of single-domain antibodies using cDNA display.
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Acknowledgments
The authors thank previous members of the Nemoto Laboratory who helped to develop this protocol, especially Dr. Yuki Mochizuki, Mr. Shinnosuke Kimura, and Mr. Takeru Suzuki. This work was supported by JSPS KAKENHI Grant Number T15H05723 and Brain and Body System Science Institute (BBSSI), Saitama University.
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Nemoto, N., Kumachi, S., Arai, H. (2018). In Vitro Selection of Single-Domain Antibody (VHH) Using cDNA Display. In: Nevoltris, D., Chames, P. (eds) Antibody Engineering. Methods in Molecular Biology, vol 1827. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8648-4_14
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DOI: https://doi.org/10.1007/978-1-4939-8648-4_14
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