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MicroRNA Analysis Using Next-Generation Sequencing

  • Monika Kolanowska
  • Anna Kubiak
  • Krystian Jażdżewski
  • Anna WójcickaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1823)

Abstract

Next-generation sequencing (NGS) enables the analysis of both microRNA expression and sequence, allowing for elucidation of a comprehensive landscape of miRNAs in a given tissue and sample type. NGS analysis requires high-quality RNA extraction and preparation of microRNA libraries. In this chapter, we describe the methods used for RNA extraction from tissue specimens, serum, cytological slides, and formalin-fixed paraffin-embedded samples. Although the described library preparation and sequencing approaches are based on Illumina NextSeq 500 sequencing technology, the presented principles shall be compatible with other commercially available sequencing platforms.

Keywords

Next-generation sequencing NGS microRNA identification microRNA quantification RNA extraction FFPE samples Serum samples 

References

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Monika Kolanowska
    • 1
    • 2
    • 3
  • Anna Kubiak
    • 1
    • 2
    • 3
  • Krystian Jażdżewski
    • 1
    • 2
    • 3
  • Anna Wójcicka
    • 1
    • 2
    Email author
  1. 1.Warsaw Genomics INCWarsawPoland
  2. 2.Genomic MedicineMedical University of WarsawWarsawPoland
  3. 3.Centre of New TechnologiesUniversity of WarsawWarsawPoland

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