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Rho GTPases pp 393-399 | Cite as

Reconstruction of ROP GTPase Domains on the Plasma Membrane in Tobacco Leaves

  • Yoshihisa OdaEmail author
  • Yoshinobu Nagashima
  • Hiroo Fukuda
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1821)

Abstract

Rho-type small GTPases (Rho GTPases) play central roles in various cellular events. Rho GTPases are often activated locally on the plasma membrane, forming plasma membrane domains, which induce downstream signaling. We describe an experimental procedure designed for inducing the production of de novo plasma membrane domains using Arabidopsis ROP11 GTPase. Introduction of ROP11 and its activator and inactivator into the tobacco leaf epidermis leads to formation of ROP11-activated plasma membrane domains on the plasma membrane. Effectors and marker genes can also be introduced alongside ROP11. This reconstruction system allows identifying molecules regulating Rho GTPase polarization.

Key words

ROP GTPase ROPGAP ROPGEF Nicotiana benthamiana Microtubule Plant Xylem Cell wall 

Notes

Acknowledgments

This work was supported by grants from MEXT KAKENHI (grant no. 16H01247 to YO and 15H05958 to HF), the JSPS KAKENHI (grant no. 16H06172 to YO and 16H06377 to HF), and the Naito Foundation to HF. YN is a special joint researcher of the National Institute of Genetics.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Yoshihisa Oda
    • 1
    • 2
    Email author
  • Yoshinobu Nagashima
    • 1
    • 3
  • Hiroo Fukuda
    • 3
  1. 1.Center for Frontier ResearchNational Institute of GeneticsShizuokaJapan
  2. 2.Department of GeneticsSOKENDAI (Graduate University for Advanced Studies)ShizuokaJapan
  3. 3.Department of Biological Sciences, Graduate School of ScienceThe University of TokyoTokyoJapan

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