Rho GTPases pp 257-266 | Cite as

Cell Polarity and Division Symmetry Analyses in Transformed Blood Cells

  • Benjamin Mizukawa
  • Eric O’Brien
  • James C. Mulloy
  • Yi ZhengEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1821)


Cdc42 was originally discovered as a key regulator of bud site assembly and polarity in S. cerevisiae. Recent genetic studies have shown that the function of Cdc42 in regulating cell polarity appears highly conserved from budding yeast to humans. The role of Cdc42 in hematopoietic cell transformation and leukemia progression has been studied in an acute myeloid leukemia model using the MLL-AF9 oncogene-induced transformation and a Cdc42 conditional gene-targeted mouse model. Here we describe the leukemia cell polarity and division symmetry assays in the context of leukemia cell fate determination.

Key words

Cell fate Cell polarity Differentiation Division symmetry Self-renewal 



The work was supported in part by NIH grants R01 CA204895, R01 CA193350, K12 HD028827, and K12 HD000850, and a St. Baldrick’s Foundation Scholar Award (BM).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Benjamin Mizukawa
    • 1
  • Eric O’Brien
    • 1
  • James C. Mulloy
    • 1
  • Yi Zheng
    • 1
    Email author
  1. 1.Cancer & Blood Diseases Institute, Cincinnati Children’s Hospital Medical CenterUniversity of CincinnatiCincinnatiUSA

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