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Olfactory Receptors pp 157–167Cite as

Long-Term Plasticity at the Mitral and Tufted Cell to Granule Cell Synapse of the Olfactory Bulb Investigated with a Custom Multielectrode in Acute Brain Slice Preparations

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1820))

Abstract

Single extracellular stimulation electrodes are a widespread means to locally activate synaptic inputs in acute brain slices. Here we describe the fabrication and application of a multielectrode stimulator that was developed for conditions under which independent stimulation of several nearby sites is desirable. For the construction of the multielectrode we have developed a method by which electrode wires can be spaced at minimal distances of 100 μm. This configuration increases the efficiency of stimulation paradigms, such as the comparison of proximal induced and control inputs for studies of synaptic plasticity.

In our case the multielectrode was used for acute olfactory bulb slices to independently excite individual nearby glomeruli; the technique allowed us to demonstrate homosynaptic bidirectional long-term plasticity at the mitral/tufted cell to granule cell synapse. We also describe the determinants for successful recordings of long-term plasticity at this synapse, with mechanical and electrophysiological recording stability being tantamount. Finally, we briefly discuss data analysis procedures.

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Acknowledgments

The construction technique of the microelectrode was established in close collaboration with the mechanical workshop at Ludwig-Maximilians-Universität München (Robert Waberer). We thank Günes Birdal for the electrode photographs in Fig. 1a, b. This work was supported by DFG grants (EG135/2, EG135/4) to V.E., the Priority Program 1665 of the German Research Foundation (HO 2156/3-1/2 to KH), the Collaborative Research Center/Transregio 166 of the German Research Foundation (B3 to KH), and the Research Group FO 1738 (WI 830/10-2 to KH).

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Authors and Affiliations

  1. Institute of Zoology and Regensburg Center of Neuroscience, Regensburg University, Regensburg, Germany

    Michael Lukas & Veronica Egger

  2. Hans-Berger Department of Neurology, University Hospital Jena, Jena, Germany

    Knut Holthoff

Authors
  1. Michael Lukas
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  2. Knut Holthoff
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  3. Veronica Egger
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Corresponding author

Correspondence to Veronica Egger .

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Editors and Affiliations

  1. Centro de Matemática Computação e Cognição (CMCC), Universidade Federal do ABC, São Bernardo do Campo, São Paulo, Brazil

    Fabio Marques Simoes de Souza

  2. Centro de Matemática Computação e Cognição (CMCC), Universidade Federal do ABC, São Bernardo do Campo, São Paulo, Brazil

    Gabriela Antunes

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Lukas, M., Holthoff, K., Egger, V. (2018). Long-Term Plasticity at the Mitral and Tufted Cell to Granule Cell Synapse of the Olfactory Bulb Investigated with a Custom Multielectrode in Acute Brain Slice Preparations. In: Simoes de Souza, F., Antunes, G. (eds) Olfactory Receptors. Methods in Molecular Biology, vol 1820. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8609-5_13

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  • DOI: https://doi.org/10.1007/978-1-4939-8609-5_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8608-8

  • Online ISBN: 978-1-4939-8609-5

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