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Odor-Induced Electrical and Calcium Signals from Olfactory Sensory Neurons In Situ

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Part of the Methods in Molecular Biology book series (MIMB, volume 1820)

Abstract

Electrophysiological recording and optical imaging enable the characterization of membrane and odorant response properties of olfactory sensory neurons (OSNs) in the nasal neuroepithelium. Here we describe a method to record the responses of mammalian OSNs to odorant stimulations in an ex vivo preparation of intact olfactory epithelium. The responses of individual OSNs with defined odorant receptor types can be monitored via patch-clamp recording or calcium imaging.

Key words

Olfactory sensory neurons Electrophysiology Patch-clamp Gene targeting Transduction Calcium imaging GCaMP6 
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Notes

Acknowledgments

M.M. is supported by the National Institute of Deafness and Other Communication Disorders, National Institute of Health (R01DC006213) and the National Science Foundation (1515930). X.G. is supported by CNRS (ATIP and ATIP-Plus grant), the Conseil Régional Bourgogne-Franche-Comté (PARI grant) and the FEDER (European Funding for Regional Economical Development).

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Centre des Sciences du Goût et de l’AlimentationUMR AgroSup, CNRS, INRA, Université de Bourgogne-Franche-ComtéDijonFrance
  2. 2.Department of NeuroscienceUniversity of Pennsylvania Perelman School of MedicinePhiladelphiaUSA

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