Abstract
Fluorescence photoactivation provides a strategy for monitoring protein kinetics within living cells. In particular, fluorescence photoactivation of a subpopulation of microtubule subunits within the spindle using photoactivatable fluorescent tubulin constructs has proven useful for assessing a variety of features of spindle microtubule dynamics, including poleward microtubule movement, microtubule depolymerization, and microtubule turnover, in various cellular settings. The current chapter describes a method for monitoring microtubule dynamics within the mouse egg spindle by photoactivation of photoactivatable-GFP-tubulin, followed by time-lapse confocal imaging.
Key words
- Mouse oocyte
- Photoactivatable GFP
- Microtubule dynamics
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FitzHarris, G. (2018). Monitoring Microtubule Dynamics in the Mouse Egg Using Photoactivatable-GFP-Tubulin. In: Verlhac, MH., Terret, ME. (eds) Mouse Oocyte Development. Methods in Molecular Biology, vol 1818. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8603-3_14
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DOI: https://doi.org/10.1007/978-1-4939-8603-3_14
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