Abstract
A protocol for the encapsulation in sodium alginate of granulosa cells in primary culture and co-culture of oocyte-cumulus complexes is reported. Sodium alginate forms strong gels when jellified with barium ions, allowing the self-organization of cells into a 3D structure. This method of encapsulation is simple and cheap, allowing the culture of cells in a 3-dimensional fashion.
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References
Gilchrist RB, Ritter LJ, Armstrong DT (2004) Oocyte-somatic cell interactions during follicle development in mammals. Anim Reprod Sci 82–83:443–446
Hussein TS, Thompson JG, Gilchrist RB (2006) Oocyte-secreted factors enhance oocyte developmental competence. Dev Biol 296:514–521
Pangas SA, Saudye H, Shea LD et al (2003) Novel approach for the three-dimensional culture of granulosa cell-oocyte complexes. Tissue Eng 9(5):1013–1021
Kreeger PK, Woodruff TK, Shea LD (2003) Murine granulosa cell morphology and function are regulated by a synthetic Arg-GlyAsp matrix. Mol Cell Endocrinol 205:1–10
Vigo D, Villani S, Faustini M et al (2005) A follicle-like model by granulosa cell encapsulation in a barium alginate/protamine membrane. Tissue Eng 11:709–714
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Faustini, M., Curone, G., Torre, M.L., Vigo, D. (2018). Bioencapsulation of Oocytes and Granulosa Cells. In: Baratta, M. (eds) Epithelial Cell Culture. Methods in Molecular Biology, vol 1817. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8600-2_9
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DOI: https://doi.org/10.1007/978-1-4939-8600-2_9
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8599-9
Online ISBN: 978-1-4939-8600-2
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