Abstract
Epithelial tissues are defined by their polarity and their ability to transport directionally. Thyroid is a tissue comprising functional epithelial units organized as enclosed follicles, with their luminal spaces defined by thyrocyte apices. Thus, the native arrangement of thyroid epithelia limits accessibility to the follicular space, presenting a challenge in studying transepithelial movements. This limitation can be overcome by studying thyrocytes grown as two-dimensional cultures. Herein we present methods for isolation of thyroid follicles from juvenile pigs and preparation of high-resistance, polarized cultures.
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Acknowledgement
We acknowledge the support of National Institutes of Health/National Institute of General Medical Sciences Award 1R15GM101674-01A1 (to P.F.), as well as the combined resources of the Department of Anatomy and Physiology at Kansas State University College of Veterinary Medicine and the Nanotechnology Innovation Center of Kansas State University (NICKS).
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Lillich, J.D., Fong, P. (2018). Isolation and Culture of Juvenile Pig Thyroid Follicular Epithelia. In: Baratta, M. (eds) Epithelial Cell Culture. Methods in Molecular Biology, vol 1817. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8600-2_2
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DOI: https://doi.org/10.1007/978-1-4939-8600-2_2
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