Abstract
Nanoimaging methods, atomic force microscopy (AFM) in particular, are widely used to study the interaction of biological molecules with the supported lipid bilayer (SLB), which itself is a traditional model for cellular membranes. Success in these studies is based on the availability of a stable SLB for the required observation period, which can extend several hours. The application of AFM requires that the SLB have a smooth morphology, thus enabling visualization of proteins and other molecules on its surface. Herein, we describe protocols for SLB assembly by using 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine (POPS) on a mica support. Our methodology enables us to assemble defect-free POPC and POPS SLBs that remain stable for at least 8 h. The application of such smooth and stable surfaces is illustrated by monitoring of the on-surface aggregation of amyloid proteins with the use of time-lapse AFM.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Coskun Ü, Simons K (2011) Cell membranes: the lipid perspective. Structure 19(11):1543–1548. https://doi.org/10.1016/j.str.2011.10.010
Ingólfsson HI, Melo MN, van Eerden FJ, Arnarez C, Lopez CA, Wassenaar TA, Periole X, de Vries AH, Tieleman DP, Marrink SJ (2014) Lipid organization of the plasma membrane. J Am Chem Soc 136(41):14554–14559. https://doi.org/10.1021/ja507832e
Nicolson GL (2014) The fluid-mosaic model of membrane structure: still relevant to understanding the structure, function and dynamics of biological membranes after more than 40 years. Biochim Biophys Acta 1838(6):1451–1466. https://doi.org/10.1016/j.bbamem.2013.10.019
Simons K, Toomre D (2000) Lipid rafts and signal transduction. Nat Rev Mol Cell Biol 1(1):31–39. https://doi.org/10.1038/35036052
Durr UH, Gildenberg M, Ramamoorthy A (2012) The magic of bicelles lights up membrane protein structure. Chem Rev 112(11):6054–6074. https://doi.org/10.1021/cr300061w
Ahmad Z, Shah A, Siddiq M, Kraatz H-B (2014) Polymeric micelles as drug delivery vehicles. RSC Adv 4(33):17028–17038. https://doi.org/10.1039/C3RA47370H
Akbarzadeh A, Rezaei-Sadabady R, Davaran S, Joo SW, Zarghami N, Hanifehpour Y, Samiei M, Kouhi M, Nejati-Koshki K (2013) Liposome: classification, preparation, and applications. Nanoscale Res Lett 8(1):102. https://doi.org/10.1186/1556-276x-8-102
Richter RP, Berat R, Brisson AR (2006) Formation of solid-supported lipid bilayers: an integrated view. Langmuir 22(8):3497–3505. https://doi.org/10.1021/la052687c
Pfefferkorn CM, Jiang Z, Lee JC (2012) Biophysics of alpha-synuclein membrane interactions. Biochim Biophys Acta 1818(2):162–171. https://doi.org/10.1016/j.bbamem.2011.07.032
Castellana ET, Cremer PS (2006) Solid supported lipid bilayers: from biophysical studies to sensor design. Surf Sci Rep 61(10):429–444. https://doi.org/10.1016/j.surfrep.2006.06.001
Plant AL, Brigham-Burke M, Petrella EC, O’Shannessy DJ (1995) Phospholipid/alkanethiol bilayers for cell-surface receptor studies by surface plasmon resonance. Anal Biochem 226(2):342–348
Shlyakhtenko LS, Gall AA, Lyubchenko YL (2013) Mica functionalization for imaging of DNA and protein-DNA complexes with atomic force microscopy. Methods Mol Biol 931:295–312. https://doi.org/10.1007/978-1-62703-056-4_14
Lyubchenko YL, Shlyakhtenko LS (2009) AFM for analysis of structure and dynamics of DNA and protein-DNA complexes. Methods 47(3):206–213. https://doi.org/10.1016/j.ymeth.2008.09.002
Andrecka J, Spillane KM, Ortega-Arroyo J, Kukura P (2013) Direct observation and control of supported lipid bilayer formation with interferometric scattering microscopy. ACS Nano 7(12):10662–10670. https://doi.org/10.1021/nn403367c
Acknowledgments
The work at University of Nebraska Medical Center was supported by grants from the National Institutes of Health (NIH) GM096039, GM118006, and NS101504 to Y.L.L. We thank Jean-Christophe Rochet (Purdue University) for providing us with the α-syn protein.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Lv, Z., Banerjee, S., Zagorski, K., Lyubchenko, Y.L. (2018). Supported Lipid Bilayers for Atomic Force Microscopy Studies. In: Lyubchenko, Y. (eds) Nanoscale Imaging. Methods in Molecular Biology, vol 1814. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8591-3_8
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8591-3_8
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8590-6
Online ISBN: 978-1-4939-8591-3
eBook Packages: Springer Protocols