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Time-Resolved Imaging of Bacterial Surfaces Using Atomic Force Microscopy

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Nanoscale Imaging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1814))

Abstract

Time-resolved atomic force microscopy (AFM) offers countless new modes by which to study bacterial cell physiology on relevant time scales, from mere milliseconds to hours and days on end. In addition, time-lapse AFM acts as a complementary tool to optical fluorescence microscopy (OFM), for which the combination offers a correlative link between the physical manifestation of bacterial phenotypes and molecular mechanisms obeying those principles. Herein we describe the essential materials and methods necessary for conducting time-resolved AFM and dual AFM/OFM experiments on bacteria.

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Acknowledgments

This work was supported by the Swiss National Science Foundation under grant agreement numbers 205321_134786 and 205320_152675. H.A. Eskandarian acknowledges the support of an EMBO advanced long-term fellowship (LTF 191-2014 & ALTF 750-2016).

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Authors and Affiliations

  1. School of Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland

    Haig Alexander Eskandarian, Adrian Pascal Nievergelt & Georg Ernest Fantner

  2. School of Life Sciences, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland

    Haig Alexander Eskandarian

Authors
  1. Haig Alexander Eskandarian
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  2. Adrian Pascal Nievergelt
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  3. Georg Ernest Fantner
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Corresponding author

Correspondence to Georg Ernest Fantner .

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Editors and Affiliations

  1. Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, Nebraska, USA

    Yuri L. Lyubchenko

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Eskandarian, H.A., Nievergelt, A.P., Fantner, G.E. (2018). Time-Resolved Imaging of Bacterial Surfaces Using Atomic Force Microscopy. In: Lyubchenko, Y. (eds) Nanoscale Imaging. Methods in Molecular Biology, vol 1814. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8591-3_23

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  • DOI: https://doi.org/10.1007/978-1-4939-8591-3_23

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8590-6

  • Online ISBN: 978-1-4939-8591-3

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