UPLC-Orbitrap® Screening for over 35 Drugs of Abuse and Metabolites in Biological Fluids in Under 10 min

  • Eshwar JagerdeoEmail author
  • Jason E. Schaff
Part of the Methods in Molecular Biology book series (MIMB, volume 1810)


We present a UPLC®-High Resolution Mass Spectrometric method to simultaneously screen for 19 benzodiazepines, 12 opiates, cocaine and three metabolites, and 3 “Z-drug” hypnotic sedatives in both blood and urine specimens. Sample processing consists of a high-speed, high-temperature enzymatic hydrolysis for urine samples followed by a rapid supported liquid extraction (SLE). The combination of ultrahigh-resolution chromatography with high resolution mass spectrometry allows all analytes to be uniquely detected with a 10 min analytical run. Limits of detection for all target analytes are 3 ng/mL or better, with only 300 μL of specimen used for analysis. The combination of low sample volume with fast processing and analysis makes this method a suitable replacement for immunoassay screening of the targeted drug classes, while providing far superior specificity and better limits of detection than can routinely be obtained by immunoassay.

Key words

Drugs of abuse Screening procedure Orbitrap™ UPLC® LC-MS Supported liquid extraction (SLE) Benzodiazepines Opiates “Z-drug” Cocaine and metabolites 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Federal Bureau of Investigation LaboratoryQuanticoUSA

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