LC-MS-MS Method Development and Analysis of Stimulants, Opiates, Synthetic Opiates, PCP, and Benzodiazepines in Wastewater. Preponderance of these Drugs During Football Games
A method was developed for the analysis of stimulant drugs, opiates, synthetic opiates, PCP, and benzodiazepines in wastewater samples using liquid chromatography coupled with tandem mass spectrometry (LC-MS-MS). A total of 33 compounds (stimulant-type drugs and metabolites of opiates, synthetic opiates, PCP, and benzodiazepines) were analyzed. These drugs included amphetamine (Amp) (1), methamphetamine (Meth) (2), methylenedioxyamphetamine (MDA) (3), methylenedioxymethamphetamine (MDMA) (4), methylenedioxyethylamphetamine (MDEA) (5), benzoylecgonine (BE, the major metabolite of Coc) (6), cocaine (Coc) (7), 6-monoacetylmorphine (6-MAM, the primary urinary metabolite of heroin) (8), codeine (9), hydrocodone (10), hydromorphone (11), morphine (12), norhydrocodone (the primary urinary metabolite of hydrocodone) (13), oxycodone (14), oxymorphone (15), 2-ethylidine-1,5-dimethyl-3,3-diphenylpyrolidine (EDDP, the primary urinary metabolite of methadone) (16), fentanyl (17), meperidine (18), methadone (19), norfentanyl (the primary urinary metabolite of fentanyl) (20), normeperidine (the primary urinary metabolite of meperidine) (21), phencyclidine (PCP) (22), tramadol (23), alprazolam (24), temazepam (25), nordiazepam (26), chlordiazepoxide (27), flurazepam (28), oxazepam (29), α-OH-alprazolam (the primary urinary metabolite of alprazolam) (30), α-OH-triazolam (the primary urinary metabolite of triazolam) (31), 2-OH-ethylflurazepam (the primary urinary metabolite of flurazepam) (32), and 7-NH2-flunitrazepam (the primary urinary metabolite of flunitrazepam) (33). These drugs were chosen because of their widespread abuse. Wastewater samples were collected at both the Oxford Wastewater Treatment Plant in Oxford, Mississippi (MS), and the University Wastewater Treatment Plant in University, MS. Samples were collected on weekends on which the Ole Miss Rebel football team held home games (Vaught-Hemingway Stadium, University, MS 38677). The collected samples were analyzed using a validated method and found to contain Amp, Meth, MDMA, MDA, Coc, BE, codeine, hydrocodone, hydromorphone, morphine, norhydrocodone, oxycodone, oxymorphone, tramadol, EDDP, meperidine, normeperidine, methadone, alprazolam, α-OH-alprazolam, nordiazepam, oxazepam, and temazepam. None of the samples contained MDEA, 6-MAM, fentanyl, norfentanyl, PCP, chlordiazepoxide, flurazepam, 2-OH-ethylflurazepam, 7-NH2-flunitrazepam, and α-OH-triazolam.
Key wordsWastewater Benzodiazepines Opiates Stimulant drugs LC-MS/MS
The authors are thankful to Mr. Randy McCluskey and employees of the Oxford Wastewater Treatment Plant, as well as Mr. David Adkisson and employees of the University Wastewater Treatment Plant for their assistance in collecting samples. The authors are also thankful to Kareem ElSohly, Candice Tolbert, Racheal Speltz, Iram Shahzadi, and Justin Reid (ElSohly Laboratories, Inc.) for their assistance.
- 1.Daughton CG (2001) Illicit drugs in municipal sewage. Proposed new nonintrusive tool to heighten public awareness of societal use of illicit-abused drugs and their potential for ecological consequences. Am Soc Symp Ser 791:348–364Google Scholar
- 2.Zuccato E, Chiabrando C, Castiglioni S, Calamari D, Bagnati R, Schiarea S et al (2005) Cocaine in surface waters: a new evidence-based tool to monitor community drug abuse. Environ Health Global Access Sci Source 4:14Google Scholar
- 7.Panawennage D, Castiglioni S, Zuccato E, Davoli E, Chiarelli MP (2011) Measurement of illicit drug consumption in small populations: prognosis for noninvasive drug testing for student populations. In: Castiglioni S, Zuccato E, Fanelli R (eds) Illicit drugs in the environment: occurrence, analysis, and fate using mass spectrometry. Chapter 18. Wiley, New Jersey, pp 321–331CrossRefGoogle Scholar
- 11.Bijlsma L, Sancho J, Pitarch E, Ibáñez M, Hernández F (2009) Simultaneous ultra-high-pressure liquid chromatography-tandem mass spectrometry determination of amphetamine and amphetamine-like stimulants, cocaine and its metabolites, and a cannabis metabolite in surface water and urban wastewater. J Chromatogr A 1216:3078–3089CrossRefPubMedGoogle Scholar
- 18.Stamper BJ, Gul W, Godfrey M, Gul S, ElSohly MA (2016) LC-MS-MS method for analysis of miscellaneous drugs in wastewater during football games III. J Forensic Toxicol 40(8):694–699Google Scholar