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Proximity Biotinylation for Studying G Protein-Coupled Receptor Dimerization

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Receptor-Receptor Interactions in the Central Nervous System

Part of the book series: Neuromethods ((NM,volume 140))

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Abstract

The importance of G protein-coupled receptor (GPCR) receptor–receptor interactions in regulating cell signaling is well documented. However, traditional methods to study these interactions are difficult to multiplex and often require extensive technical expertise and equipment. In this chapter, we will describe the major biochemical and fluorescence microscopy methods to study GPCR–GPCR interactions and introduce an alternative technique that utilizes proximity biotinylation to study these interactions. The goal of this chapter is to provide researchers with a comprehensive protocol to implement this new approach to study GPCR dimerization. Finally, we will compare and contrast the advantages and limitations of the proximity biotinylation assay with established methods to help researchers identify the best approach for their research application.

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Author information

Authors and Affiliations

  1. Laboratory of Cellular and Molecular Systems, Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI, USA

    Maxwell S. DeNies, Luciana K. Rosselli-Murai, Victoria L. Murray, Elisabeth M. Steel & Allen P. Liu

Authors
  1. Maxwell S. DeNies
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  2. Luciana K. Rosselli-Murai
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  3. Victoria L. Murray
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  4. Elisabeth M. Steel
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  5. Allen P. Liu
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Corresponding author

Correspondence to Allen P. Liu .

Editor information

Editors and Affiliations

  1. Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden

    KJELL FUXE

  2. Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden

    Dasiel O. Borroto-Escuela

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DeNies, M.S., Rosselli-Murai, L.K., Murray, V.L., Steel, E.M., Liu, A.P. (2018). Proximity Biotinylation for Studying G Protein-Coupled Receptor Dimerization. In: FUXE, K., Borroto-Escuela, D. (eds) Receptor-Receptor Interactions in the Central Nervous System. Neuromethods, vol 140. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8576-0_16

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  • DOI: https://doi.org/10.1007/978-1-4939-8576-0_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8575-3

  • Online ISBN: 978-1-4939-8576-0

  • eBook Packages: Springer Protocols

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