Abstract
Ozone is a major pollutant in the air we breathe, and elevated levels lead to significant morbidity and mortality. As the climate warms, levels of ozone are predicted to increase. Accordingly, studies to assess the mechanisms of ozone-induced lung diseases are paramount. This chapter describes mouse models of ozone exposure and methods for assessing the effects of ozone in the lungs. These include bronchoalveolar lavage, necropsy, and measurement of lung function. Lavage allows for assessment of cell infiltration, cytokine production, tissue damage and capillary leakage in the airspaces. Necropsy provides tissue for gene expression, histology, and protein assessment in the whole lung. Lung physiology is used to assess airway hyperresponsiveness, tissue and total lung resistance, compliance, and elastance.
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References
Dockery DW, Pope CA 3rd, Xu X, Spengler JD, Ware JH, Fay ME, Ferris BG Jr, Speizer FE (1993) An association between air pollution and mortality in six U.S. cities. N Engl J Med 329(24):1753–1759. https://doi.org/10.1056/NEJM199312093292401
Bell ML, McDermott A, Zeger SL, Samet JM, Dominici F (2004) Ozone and short-term mortality in 95 US urban communities, 1987-2000. JAMA 292(19):2372–2378. https://doi.org/10.1001/jama.292.19.2372
Gryparis A, Forsberg B, Katsouyanni K, Analitis A, Touloumi G, Schwartz J, Samoli E, Medina S, Anderson HR, Niciu EM, Wichmann HE, Kriz B, Kosnik M, Skorkovsky J, Vonk JM, Dortbudak Z (2004) Acute effects of ozone on mortality from the “air pollution and health: a European approach” project. Am J Respir Crit Care Med 170(10):1080–1087. https://doi.org/10.1164/rccm.200403-333OC
Katsouyanni K, Zmirou D, Spix C, Sunyer J, Schouten JP, Ponka A, Anderson HR, Le Moullec Y, Wojtyniak B, Vigotti MA et al (1995) Short-term effects of air pollution on health: a European approach using epidemiological time-series data. The APHEA project: background, objectives, design. Eur Respir J 8(6):1030–1038
Hubbell BJ, Hallberg A, McCubbin DR, Post E (2005) Health-related benefits of attaining the 8-hr ozone standard. Environ Health Perspect 113(1):73–82
Ito K, De Leon SF, Lippmann M (2005) Associations between ozone and daily mortality: analysis and meta-analysis. Epidemiology 16(4):446–457
Levy JI, Chemerynski SM, Sarnat JA (2005) Ozone exposure and mortality: an empiric bayes metaregression analysis. Epidemiology 16(4):458–468
Fann N, Lamson AD, Anenberg SC, Wesson K, Risley D, Hubbell BJ (2012) Estimating the national public health burden associated with exposure to ambient PM2.5 and ozone. Risk Anal 32(1):81–95. https://doi.org/10.1111/j.1539-6924.2011.01630.x
Grambsch A; Hemming BL, CP. Weaver Gilliland A, Grano D, Hunt S, Johnson T, Loughlin D, Winner D (2009) Assessment of the Impacts of Global Change on Regional US Air Quality: a synthesis of climate change impacts on ground-level ozone. www.epa.gov. 2016
Cilley RE, Wang JY, Coran AG (1993) Lung injury produced by moderate lung overinflation in rats. J Pediatr Surg 28(3):488–493. discussion 494–485
Hoymann HG (2007) Invasive and noninvasive lung function measurements in rodents. J Pharmacol Toxicol Methods 55(1):16–26. https://doi.org/10.1016/j.vascn.2006.04.006
Hinners RG, Burkart JK, Punte CL (1968) Animal inhalation exposure chambers. Arch Environ Health 16(2):194–206
McGovern TK, Robichaud A, Fereydoonzad L, Schuessler TF, Martin JG (2013) Evaluation of respiratory system mechanics in mice using the forced oscillation technique. https://www.jove.com/video/50172/evaluation-respiratory-system-mechanics-mice-using-forced-oscillation. Accessed 24 July 2017
Funding
This work was supported through funding from the Division of Intramural Research, National Institute of Environmental Health Sciences. Grant number 1ZIAES102605.
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Stober, V.P., Garantziotis, S. (2018). Assessment of Ozone-Induced Lung Injury in Mice. In: Alper, S., Janssen, W. (eds) Lung Innate Immunity and Inflammation. Methods in Molecular Biology, vol 1809. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8570-8_19
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DOI: https://doi.org/10.1007/978-1-4939-8570-8_19
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