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Mass Spectrometry of Gangliosides

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Book cover Gangliosides

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1804))

Abstract

This chapter describes protocols for mass spectrometry (MS) applied to the characterization of ganglioside structures and the determination of ganglioside contents. Matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) are often used to ionize biological materials and this chapter covers three protocols for atmospheric pressure MALDI MS (AP-MALDI MS), liquid chromatography-ESI MS (LC-ESI MS), and LC-ESI MS with multiple reaction monitoring (MRM). Purified gangliosides were used in AP-MALDI MS analyses while crude preparations of gangliosides were subjected to LC-ESI MS and LC-ESI MS with MRM. The LC protocol includes conditions for both reversed-phase and normal-phase column chromatography.

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Author information

Authors and Affiliations

  1. Division of Glycopathology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai, Miyagi, Japan

    Akemi Suzuki, Takahiro Nitta & Jin-ichi Inokuchi

  2. School of Integrative and Global Majors, Tsukuba University, Tsukuba, Ibaraki, Japan

    Minoru Suzuki

  3. Brain Research Center, RIKEN, Wakō, Saitama, Japan

    Emi Ito

Authors
  1. Akemi Suzuki
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  2. Minoru Suzuki
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  3. Emi Ito
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  4. Takahiro Nitta
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  5. Jin-ichi Inokuchi
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Corresponding author

Correspondence to Akemi Suzuki .

Editor information

Editors and Affiliations

  1. Department of Medical Biotech & Translational Medicine, University of Milan, Segrate, Milano, Italy

    Sandro Sonnino

  2. Department of Medical Biotech & Translational Medicine, University of Milan, Segrate, Milano, Italy

    Alessandro Prinetti

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Suzuki, A., Suzuki, M., Ito, E., Nitta, T., Inokuchi, Ji. (2018). Mass Spectrometry of Gangliosides. In: Sonnino, S., Prinetti, A. (eds) Gangliosides. Methods in Molecular Biology, vol 1804. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8552-4_9

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  • DOI: https://doi.org/10.1007/978-1-4939-8552-4_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8551-7

  • Online ISBN: 978-1-4939-8552-4

  • eBook Packages: Springer Protocols

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