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Gangliosides pp 429–435Cite as

Identification of KDN-Gangliosides

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1804))

Abstract

KDN is an abbreviated name of 2-keto-3-deoxy-d-glycero-d-galacto-nononic acid, and belongs to sialic acid members like N-acetylneuraminic acid (Neu5Ac), and N-glycolylneuraminic acid (Neu5Gc). The aminoacyl group at C5 position of Neu5Ac is replaced by a hydroxyl group in KDN. Like Neu5Ac, KDN exists in various glycoconjugates including glycosphingolipids in vertebrates and gram-negative bacteria. Because of its unique properties, some methods are specifically applicable to KDN residue, although most detection methods for Neu5Ac are also applicable. In this chapter, methods for identification of KDN residues in glycosphingolipids are described, focusing on two methods that are often used, i.e., the fluorescent HPLC analysis and the TLC immunostaining with the antibodies specific to α2,3- and α2,8-KDN residues.

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Authors and Affiliations

  1. Bioscience and Biotechnology Center, Nagoya University, Nagoya, Japan

    Ken Kitajima

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  1. Ken Kitajima
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Correspondence to Ken Kitajima .

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Editors and Affiliations

  1. Department of Medical Biotech & Translational Medicine, University of Milan, Segrate, Milano, Italy

    Sandro Sonnino

  2. Department of Medical Biotech & Translational Medicine, University of Milan, Segrate, Milano, Italy

    Alessandro Prinetti

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Kitajima, K. (2018). Identification of KDN-Gangliosides. In: Sonnino, S., Prinetti, A. (eds) Gangliosides. Methods in Molecular Biology, vol 1804. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8552-4_22

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  • DOI: https://doi.org/10.1007/978-1-4939-8552-4_22

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8551-7

  • Online ISBN: 978-1-4939-8552-4

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