Abstract
Structure, conformation, and dynamics of sphingolipids can provide substantial help in better understanding sphingolipid–ligand interaction mechanisms. Both the oligosaccharide structure and the ceramide moiety of native glycosphingolipid can be established directly by NMR spectroscopic analysis without the necessity to resort to any other chemical or spectroscopic methods. NMR is a powerful technique to investigate interaction between small ligand, such as ganglioside, and membrane protein.
Keywords
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- 1D:
-
One-dimensional
- 2D:
-
Two-dimensional
- Cer:
-
Ceramide
- COSY:
-
Correlation spectroscopy
- DANTE:
-
Delays alternated by nutation for tailored excitation
- Deac-GM1:
-
Neu-GM1
- DEPT:
-
Distortionless enhancement by polarization transfer
- DMPC:
-
1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine
- DMPE:
-
Dimyristoyl phosphatidylethanolamine
- DMPG:
-
1,2-Dimyristoyl-sn-glycero-3-phosphoglycerol
- DMPS:
-
Dimyristoyl phosphatidylserine
- DMSO:
-
Dimethyl-d 6 -sulfoxide
- DOSY:
-
Diffusion Ordered Spectroscopy
- DPC:
-
Dodecylphosphocholine
- DPC:
-
Dodecylphosphocholine-d38;
- EDTA:
-
Ethylenediaminetetraacetic acid
- FA:
-
Fatty acid
- Gal:
-
Galactose
- GalNAc:
-
N-acetylgalactosamine
- Glc:
-
Glucose
- HMBC:
-
Heteronuclear Multiple Bond Correlation
- HOHAHA:
-
Homonuclear Hartman Hahn
- HSQC:
-
Heteronuclear Single Quantum Coherence
- INEPT:
-
Insensitive nuclei enhanced by polarization transfer
- MD:
-
Molecular dynamics
- MINSY:
-
Mixing irradiation during NOESY
- MLEV:
-
Malcolm LEVitt's decoupling cycle
- Neu5Ac:
-
N-acetylneuraminic acid
- Neu5Gc:
-
Neu5Gc N-glycolylneuraminic acid
- NMR:
-
Nuclear Magnetic Resonance
- NOE:
-
Nuclear Overhauser effect
- NOESY:
-
NOE correlated spectroscopy
- PCS:
-
Pseudocontact shift
- PFG:
-
Pulsed Field Gradient
- PFG-SE:
-
Pulsed Field Gradient Spin Echo
- PRE:
-
Paramagnetic Relaxation Enhancement
- ROE:
-
Rotating-frame nuclear Overhauser effect
- ROESY:
-
Rotating-frame Overhauser Effect Spectroscopy
- Sph:
-
Sphingosine
- STD:
-
Saturation transfer difference
- STEP:
-
Selective TOCSY edited preparation
- SUV:
-
Small Unilamellar Vesicles
- TOCSY:
-
Total correlated spectroscopy
- TPPI:
-
Time proportional phase incrementation
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Acquotti, D., Mauri, L., Sonnino, S. (2018). Nuclear Magnetic Resonance of Gangliosides. In: Sonnino, S., Prinetti, A. (eds) Gangliosides. Methods in Molecular Biology, vol 1804. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8552-4_12
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