Abstract
Delayed-type hypersensitivity (DTH or type IV hypersensitivity) is defined by T-cell-driven inflammation which occurs only after secondary insult with antigen. Interestingly both Th1, the classical, and Th17, the more recently discovered, proinflammatory lineages have been implicated in disease progression. The duality of DTH makes it an ideal model system for understanding T-cell differentiation, memory cell formation, and the direct effect of treatment regimens on T-cell activation/proliferation. To this end, a protocol for induction and assessment of DTH which triggers memory T-cell (Th1 and Th17) response in the footpad of mice using methylated bovine serum albumin (mBSA) is described.
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References
Gell PGH, Coombs RRA (1963) The classification of allergic reactions underlying disease. In: Clinical aspects of immunology. Blackwell Science, Copenhagen
Brostoff J, Scadding GK, Male D, Roitt IM (1991) Introduction to immune responses. In: Clinical Immunology. Gower Medical Publishing, New York
Fong TA, Mosmann TR (1989) The role of IFN-gamma in delayed-type hypersensitivity mediated by Th1 clones. J Immunol 143:2887–2893
Nakae S, Komiyama Y, Nambu A, Sudo K, Iwase M, Homma I, Sekikawa K, Asano M, Iwakura Y (2002) Antigen-specific T cell sensitization is impaired in IL-17-deficient mice, causing suppression of allergic cellular and humoral responses. Immunity 17:375–387
Manousek J, Stejskal V, Kubena P, Jarkovsky J, Nemec P, Lokaj P, Dostalova L, Zadakova A, Pavlusova M, Benesova K, Kala P, Miklik R, Spinar J, Parenica J (2016) Delayed-type hypersensitivity to metals of environmental burden in patients with Takotsubo Syndrome – is there a clinical relevance? PLoS One 11:e0164786. https://doi.org/10.1371/journal.pone.0164786. eCollection 2016
Cotton CH, Admani SE, Jacob SE, Krakowski AC (2016) “Mint” condition: contact dermatitis in an adolescent numismatist. Pediatr Dermatol 33:80–83
Hanson JL, Warshaw EM (2015) Contact allergy to surfactants in a hypoallergenic liquid cleanser. Dermatitis 26(6):284
Nosbaum A, Vocanson M, Rozieres A, Hennino A, Nicolas JF (2009) Allergic and irritant contact dermatitis. Eur J Dermatol 19:325–332
Jackson AR, Nagarkatti P, Nagarkatti M (2014) Anandamide attenuates Th-17 cell-mediated delayed-type hypersensitivity response by triggering IL-10 production and consequent microRNA induction. PLoS One 9:e93954. https://doi.org/10.1371/journal.pone.0093954. eCollection 2014
Sido JM, Jackson AR, Nagarkatti PS, Nagarkatti M (2016) Marijuana-derived Δ-9-tetrahydrocannabinol suppresses Th1/Th17 cell-mediated delayed-type hypersensitivity through microRNA regulation. J Mol Med (Berl) 94(9):1039–1051
Acknowledgments
I wish to acknowledge Dr. Austin R. Jackson (University of South Carolina School of Medicine) for the use of his histology images.
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Sido, J.M. (2018). Methylated Bovine Serum Albumin (mBSA)-Induced Delayed-Type Hypersensitivity in Mice. In: DeWitt, J., Rockwell, C., Bowman, C. (eds) Immunotoxicity Testing. Methods in Molecular Biology, vol 1803. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8549-4_7
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DOI: https://doi.org/10.1007/978-1-4939-8549-4_7
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