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Functional Assays of Hematopoietic Stem Cells in Toxicology Research

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Immunotoxicity Testing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1803))

Abstract

The hematopoietic stem cell is the foundational cell of the entire blood and immune system and as such is particularly sensitive to toxicological insults. While this review will identify some of the classes of chemicals known to be hematotoxic, most of the discussion will focus on the strengths and weaknesses of various hematological assays used in toxicology research. Furthermore, protocols for isolating both human and murine hematopoietic stem cells are described. Methodologies are also described for various culture systems useful for testing the impacts of potential toxicants on hematopoietic stem cells both in vivo and in vitro.

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Author information

Authors and Affiliations

  1. Joseph J. Zilber School of Public Health, University of Wisconsin—Milwaukee, Milwaukee, WI, USA

    Michael D. Laiosa

Authors
  1. Michael D. Laiosa
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Corresponding author

Correspondence to Michael D. Laiosa .

Editor information

Editors and Affiliations

  1. Department of Pharmacology and Toxicology, East Carolina University, Greenville, North Carolina, USA

    Jamie C. DeWitt

  2. Department of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan, USA

    Cheryl E. Rockwell

  3. Durham, North Carolina, USA

    Christal C. Bowman

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Laiosa, M.D. (2018). Functional Assays of Hematopoietic Stem Cells in Toxicology Research. In: DeWitt, J., Rockwell, C., Bowman, C. (eds) Immunotoxicity Testing. Methods in Molecular Biology, vol 1803. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8549-4_19

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  • DOI: https://doi.org/10.1007/978-1-4939-8549-4_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8548-7

  • Online ISBN: 978-1-4939-8549-4

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