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Dendritic Cell Assays

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Immunotoxicity Testing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1803))

Abstract

Generation of dendritic cells from both mouse and human tissues is a valuable technique for downstream immunotoxicological applications. Here, we describe methods for generation of four subsets of dendritic cells from murine bone marrow and three subsets of dendritic cells from human peripheral blood mononuclear cells.

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References

  1. Merad M, Sathe P, Helft J, Miller J, Mortha A (2013) The dendritic cell lineage: ontogeny and function of dendritic cells and their subsets in the steady state and the inflamed setting. Annu Rev Immunol 31:563–604

    Article  CAS  PubMed  Google Scholar 

  2. Mildner A, Jung S (2014) Development and function of dendritic cell subsets. Immunity 40(5):642–656

    Article  CAS  PubMed  Google Scholar 

  3. Geissmann F, Auffray C, Palframan R, Wirrig C, Ciocca A, Campisi L, Narni-Mancinelli E, Lauvau G (2008) Blood monocytes: distinct subsets, how they relate to dendritic cells, and their possible roles in the regulation of T-cell responses. Immunol Cell Biol 86(5):398–408

    Article  CAS  PubMed  Google Scholar 

  4. Simones T, Shepherd DM (2010) Consequences of AhR activation in steady-state dendritic cells. Toxicol Sci 119(2):293–307

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  5. Bankoti J, Rase B, Simones T, Shepherd DM (2010) Functional and phenotypic effects of AhR activation in inflammatory dendritic cells. Toxicol Appl Pharmacol 246(1):18–28

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  6. Gilliet M, Boonstra A, Paturel C, Antonenko S, Xu X-L, Trinchieri G, O'Garra A, Liu Y-J (2002) The development of murine plasmacytoid dendritic cell precursors is differentially regulated by FLT3-ligand and granulocyte/macrophage colony-stimulating factor. J Exp Med 195(7):953–958

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  7. Lutz MB, Kukutsch N, Ogilvie AL, Rößner S, Koch F, Romani N, Schuler G (1999) An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow. J Immunol Methods 223(1):77–92

    Article  PubMed  CAS  Google Scholar 

  8. Katz SI, Tamaki K, Sachs DH (1979) Epidermal Langerhans cells are derived from cells originating in bone marrow. Nature 282:324–326

    Article  PubMed  CAS  Google Scholar 

  9. Ohtsuka H, Sakamoto A, Pan J, Inage S, Horigome S, Ichii H, Arima M, Hatano M, Okada S, Tokuhisa T (2011) Bcl6 is required for the development of mouse CD4+ and CD8α+ dendritic cells. J Immunol 186(1):255–263

    Article  PubMed  CAS  Google Scholar 

  10. Shortman K, Naik SH (2007) Steady-state and inflammatory dendritic-cell development. Nat Rev Immunol 7(1):19–30

    Article  PubMed  CAS  Google Scholar 

  11. Vremec D, Pooley J, Hochrein H, Wu L, Shortman K (2000) CD4 and CD8 expression by dendritic cell subtypes in mouse thymus and spleen. J Immunol 164(6):2978–2986

    Article  PubMed  CAS  Google Scholar 

  12. Geissmann F, Prost C, Monnet J-P, Dy M, Brousse N, Hermine O (1998) Transforming growth factor β1, in the presence of granulocyte/macrophage colony-stimulating factor and interleukin 4, induces differentiation of human peripheral blood monocytes into dendritic Langerhans cells. J Exp Med 187(6):961–966

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  13. Dauer M, Obermaier B, Herten J, Haerle C, Pohl K, Rothenfusser S, Schnurr M, Endres S, Eigler A (2003) Mature dendritic cells derived from human monocytes within 48 hours: a novel strategy for dendritic cell differentiation from blood precursors. J Immunol 170(8):4069–4076

    Article  PubMed  CAS  Google Scholar 

  14. Borras F, Matthews N, Patel R, Navarrete C (2000) Dendritic cells can be successfully generated from CD34+ cord blood cells in the presence of autologous cord blood plasma. Bone Marrow Transplant 26(4):371–376

    Article  PubMed  CAS  Google Scholar 

  15. Tkachenko N, Wojas K, Tabarkiewicz J, Rolinski J (2005) Generation of dendritic cells from human peripheral blood monocytes-comparison of different culture media. Folia Histochem Cytobiol 43(1):25–30

    PubMed  CAS  Google Scholar 

  16. Massa C, Thomas C, Wang E, Marincola F, Seliger B (2015) Different maturation cocktails provide dendritic cells with different chemoattractive properties. J Transl Med 13:175

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  17. Zarif JCHJ, Verdone JE, Campbell SP, Drake CG, Pienta K (2016) A phased strategy to differentiate human CD14+monocytes into classically and alternatively activated macrophages and dendritic cells. BioTechniques 61(1):33–41

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  18. Hulspas R, O’Gorman MRG, Wood BL, Gratama JW, Sutherland DR (2009) Considerations for the control of background fluorescence in clinical flow cytometry. Cytometry 76B:355–364

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by NIH grant ES013784 (to D.M.S.) and an AAI Careers in Immunology Fellowship (to J.M.K.).

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Authors and Affiliations

  1. Cellular, Molecular, and Microbial Biology Graduate Program, Division of Biological Sciences, University of Montana, Missoula, MT, USA

    Joanna M. Kreitinger

  2. Department of Biomedical and Pharmaceutical Sciences, Center for Translational Medicine, University of Montana, Missoula, MT, USA

    David M. Shepherd

Authors
  1. Joanna M. Kreitinger
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  2. David M. Shepherd
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Corresponding author

Correspondence to David M. Shepherd .

Editor information

Editors and Affiliations

  1. Department of Pharmacology and Toxicology, East Carolina University, Greenville, North Carolina, USA

    Jamie C. DeWitt

  2. Department of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan, USA

    Cheryl E. Rockwell

  3. Durham, North Carolina, USA

    Christal C. Bowman

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Kreitinger, J.M., Shepherd, D.M. (2018). Dendritic Cell Assays. In: DeWitt, J., Rockwell, C., Bowman, C. (eds) Immunotoxicity Testing. Methods in Molecular Biology, vol 1803. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8549-4_16

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  • DOI: https://doi.org/10.1007/978-1-4939-8549-4_16

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8548-7

  • Online ISBN: 978-1-4939-8549-4

  • eBook Packages: Springer Protocols

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