Dendritic Cell Assays

  • Joanna M. Kreitinger
  • David M. Shepherd
Part of the Methods in Molecular Biology book series (MIMB, volume 1803)


Generation of dendritic cells from both mouse and human tissues is a valuable technique for downstream immunotoxicological applications. Here, we describe methods for generation of four subsets of dendritic cells from murine bone marrow and three subsets of dendritic cells from human peripheral blood mononuclear cells.

Key words

Dendritic cell Peripheral blood mononuclear cells (PBMCs) Bone marrow-derived dendritic cells (BMDCs) In vitro Cell culture 



This work was supported by NIH grant ES013784 (to D.M.S.) and an AAI Careers in Immunology Fellowship (to J.M.K.).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Cellular, Molecular, and Microbial Biology Graduate Program, Division of Biological SciencesUniversity of MontanaMissoulaUSA
  2. 2.Department of Biomedical and Pharmaceutical Sciences, Center for Translational MedicineUniversity of MontanaMissoulaUSA

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