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Natural Killer (NK) Cell Assays in Immunotoxicity Testing

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1803)

Abstract

It is well known that natural killer (NK) cells are involved in defense against viruses and some tumors. NK cells kill target cells by the directed release of cytolytic granules that contain perforin, granzymes, and granulysin. It is increasingly important to evaluate NK cell function in immunotoxicity testing. NK cell function can be evaluated by determining cytolytic activity against target tumor cells by the 51Cr-release assay and also by determining the number of NK cells in peripheral blood in humans and in the spleen in animals using flow cytometry. Recently, the intracellular levels of perforin, granzymes, and granulysin determined by flow cytometry have also been used in the evaluation of NK cell function. In addition, chemical-induced apoptosis in NK cells also has been applied to evaluate the immunotoxicity of chemicals. This chapter will describe the methods for NK cell assays in immunotoxicity testing.

Key words

Apoptosis Chromium release Flow cytometry Granulysin Granzymes Immunotoxicity NK activity Perforin 

Notes

Acknowledgments

The parts of experimental data shown in this chapter and performed by author were supported by grants from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (No. 09877077, No. 10770178, No. 12770206, No. 15590523, No. 19590602, No. 22590554, and No. 26460811).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Hygiene and Public HealthNippon Medical SchoolTokyoJapan
  2. 2.Department of Rehabilitation and Physical MedicineGraduate School of Medicine, Nippon Medical SchoolTokyoJapan

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